果胶杆菌(Pectobacterium aroidearum)WNH的筛选及其汉麻生物脱胶效果

Screening of Pectobacterium aroidearum WNH with degumming effect on hemp

【背景】麻类生物脱胶与化学法脱胶相比具有环保优势。【目的】获得用于汉麻生物脱胶的高效果胶酶菌株。【方法】使用以果胶为唯一碳源的培养基,采用平板稀释法进行菌株筛选,通过生理生化实验和16SrRNA基因序列比对鉴定目标菌株。采用单因素试验优化产酶条件,并验证该条件下汉麻生物脱胶效果。【结果】获得一株具备高活性果胶的酶菌株,归类为果胶杆菌(Pectobacterium aroidearum)WNH。在培养温度27℃、转速160 r/min、接种量10%、初始pH7.0的条件下培养16 h后,果胶杆菌WNH的粗酶液果胶酶活力达155.03 U/mL。按上述条件对汉麻韧皮进行二次脱胶处理,处理后脱胶率为27.18%,较对照组提高了6.93%。【结论】果胶杆菌WNH具备汉麻生物脱胶的潜力。

[Background]Compared with chemical degumming,biological degumming of hemp is environmental-friendly.[Objective]To obtain an efficient pectinase-producing strain for the biological degumming of hemp.[Methods]The strains were screened by plate dilution method in the medium with pectin as the sole carbon source,and the target strain was identified by physiological and biochemical tests and 16S rRNA gene sequence alignment.The enzyme production conditions were optimized by single factor experiment,and the biological degumming effect of hemp was verified under the optimal conditions.[Results]A highly active pectinase-producing strain,Pectobacterium aroidearum WNH,was screened out.After culture for 16 h at 27℃,the rotation speed of 160 r/min,inoculum volume of 10%,and initial pH 7,the crude enzyme solution of P.aroidearum WNH showed the pectinase activity of 155.03 U/mL.Under the above conditions,the second degumming of hemp bast was carried out,and the degumming rate reached 27.18%,which was 6.93% higher than that of the control group.[Conclusion]P.aroidearum WNH has the potential for the biological degumming of hemp.