基于互穿网络技术构筑的氧化海藻酸钠/纤维素纳米晶/聚丙烯酰胺-明胶双网络水凝胶与性能

Construction and Property of Oxidized Sodium Alginate/Cellulose Nanocrystal/Polyacrylamide-Gelatin Composite Hydrogel Based on Interpenetrating Network Technology

为了提高海藻酸盐水凝胶的生物应用性,以氧化海藻酸钠(OSA)为原料,采用互穿网络技术、纤维素纳米晶(CNCs)补强和明胶(GT)表面覆积相结合的方法构建了OSA/CNCs/PAM-GT复合水凝胶(其中,PAM为聚丙烯酰胺)。通过FTIR、TGA、XRD、SEM、溶胀性和降解性实验以及细胞相容性测试考察了CNCs含量对OSA/CNCs/PAM-GT复合水凝胶结构和性能的影响。结果表明,CNCs能够与基体中的聚合物产生相互作用力。随着CNCs含量的增加,OSA/CNCs/PAM-GT复合水凝胶的孔隙率下降,力学性能提高,其溶胀率随CNCs含量增加呈下降趋势(下降幅度较小)而生物降解率呈上升趋势,说明CNCs能够在一定程度上调控复合水凝胶的物化性能。OSA/CNCs/PAM-GT复合水凝胶展现出较好的细胞黏附、增殖和分化性能。当CNCs的含量为0.5%时(以OSA溶液质量为基准,下同),人骨瘤(MG63)细胞增殖效果最佳,而CNCs的含量为1.5%时,MG63细胞分化效果最显著。

To improve the bio-applicability of alginate hydrogel,the composite hydrogels of oxidized sodium alginate(OSA)/cellulose nanocarrier(CNCs)/polyacrylamide-gelatin(PAM-GT)were constructed from combination of interpenetrating network technology,CNCs reinforcement and GT surface deposition with OSA.The effects of CNCs dosage on the structure and properties of OSA/CNCs/PAM-GT composite hydrogels were analyzed by FTIR,TGA,XRD,SEM,swelling and degradability experiments as well as cytocompatibility tests.The results showed that CNCs could interact with the polymers in the matrix.As the dosage of CNCs increased,the composite hydrogel exhibited a decreased porosity,enhanced mechanical strength and biodegradability,and a slightly reduced swelling,indicating that CNCs could regulate the physicochemical properties of the composite hydrogels to a certain extent.Meanwhile,the composite hydrogels displayed good cell adhesion,proliferation and differentiation properties.When the dosage of CNCs was 0.5%(based on the mass of OSA solution,the same below),the composite hydrogel showed the best cell proliferation activity for MG63 cells,while displayed the most significant cell differentiation activity when the dosage of CNCs was 1.5%.