尿毒症维持性血液透析患者外周血NLRP3、IL-1β、IL-18水平与血管钙化的相关性分析

Correlation Analysis of Peripheral Blood NLRP3,IL-1β,IL-18 Levels and Vascular Calcification in Uremic Maintenance Hemodialysis Patients

目的:探讨分析尿毒症维持性血液透析患者外周血NOD样受体蛋白3(NLRP3)、白细胞介素-1β(IL-1β)、白细胞介素-18(IL-18)水平与血管钙化的相关性。方法:选取2019年12月—2020年12月在我院进行维持性血液透析的86例尿毒症患者,使用腹部侧位平片评估血管钙化情况,将患者分为血管钙化组34例和无血管钙化组52例,采用酶联免疫吸附法检测其外周血NLRP3、IL-1β、IL-18水平,分析其水平变化与血管钙化的关系。结果:2组患者年龄、透析龄、血磷、超敏C反应蛋白、NLRP3、IL-1β、IL-18等差异存在统计学意义,提示其变化与血管钙化有关(P<0.05);多因素Logistic回归分析结果显示,外周血NLRP3、IL-1β、IL-18水平异常升高是尿毒症维持性血液透析患者血管钙化发生的独立影响因素(P<0.05);Pearson相关性分析结果显示,外周血NLRP3、IL-1β、IL-18水平与血管钙化成正相关关系(P<0.001)。结论:外周血NLRP3、IL-1β、IL-18水平是尿毒症血管钙化的独立影响因素,其水平与患者血管钙化程度呈正相关关系,可为血管钙化及预后评估提供指导依据。

Objective:To explore and analyze the correlation between the peripheral blood NLRP3,IL-1β,IL-18 levels and vascular calcification in patients with uremic maintenance hemodialysis.Methods:A total of 86 patients with uremic who underwent maintenance hemodialysis in our hospital from December 2019 to December 2020 were selected.Lateral abdominal radiographs were used to assess vascular calcification.The patients were divided into vascular calcification group,34 cases and avascular calcification group.In 52 cases,the levels of NOD-like receptor protein 3,interleukin-1β,and interleukin-18 in peripheral blood were detected by enzyme-linked immunosorbent assay,and the relationship between their level changes and vascular calcification was analyzed relation.Results:There were significant differences in age,dialysis age,blood phosphorus,CRP,NLRP3,IL-1β,IL-18,etc.between the two groups,suggesting that the changes were related to vascular calcification(P<0.05);the results of multivariate logistic regression analysis showed that,Abnormally elevated levels of NLRP3,IL-1β,and IL-18 in peripheral blood are independent factors influencing the occurrence of vascular calcification in patients with maintenance hemodialysis(P<0.05);Pearson correlation analysis results show that peripheral blood NLRP3,IL-1β,IL-18 levels are positively correlated with vascular calcification(P<0.001).Conclusion:Peripheral blood NLRP3,IL-1β,IL-18 levels are independent influencing factors of vascular calcification in uremia,and their levels are positively correlated with the degree of vascular calcification in patients,which can provide guidance for vascular calcification and prognosis evaluation.