藏药蕨麻的化学成分UHPLC-Q-TOF/MS分析

UHPLC-Q-TOF/MS Analysis of Chemical Constituents from Roots of Tibetan Medicine Potentilla Anserina L.

蕨麻不仅是藏区百姓的传统食品,还是藏医习用药材,本试验以西藏拉萨市蕨麻块根为原料,使用液质联用法对蕨麻进行活性成分快速定性研究。用80%甲醇进行提取,采用Waters Xevo G2-XS QTOF高分辨质谱进行分析;色谱条件为Waters BEH C18色谱柱(2.1 mm×100 mm,1.7μm),以0.1%-甲酸水溶液(A)-乙腈(B)为流动相梯度洗脱(0~2 min,5%~10%B;2~10 min,10%~20%B;10~15 min,72%~100%B;15~71 min,2%~100%B;71~72 min,2%B),流速0.3 mL/min,进样量3μL;质谱条件为电喷雾离子源(ESI),检测范围m/z 50~1000,负离子模式采集。鉴定解谱得出27个化合物,其中含五对同分异构体,负模式下则鉴定黄酮类化合物21个、三萜类化合物6个。本研究为鉴别藏药蕨麻的化学成分提供一种准确快速的方法,为蕨麻在药品与食品领域的利用开发提供理论基础。

The underground root of Potentilla anserina is the traditional food of the people in Tibetan areas,and it is also commonly used as Tibetan medicine.In this study,a rapid qualitative study by LC-MS was conducted on the active components of Potentilla anserina tuber root in Lhasa,Tibet.The tuber root was extracted by 80%methanol and analyzed by Waters Xevo G2-XS QTOF high resolution mass spectrometry.The chromatographic conditions were Waters BEH C18 column(2.1×100mm,1.7um)with gradient elution of 0.1%formic acid(A)-acetonitrile(B)as mobile phase(0~2 min,5%-10%B;2~10 min,10%-20%B;10~15 min,72%-100%B;15-71 min,2%-100%B;71-72 min,2%B),the flow rate 0.3 mL/min,the sample size 3μL.The mass spectrum conditions were electrospray ion source(ESI),detection range m/z 50~1000,negative ion mode acquisition.Twenty-seven compounds were identified,including five pairs of isomers.Under negative mode,twenty-one flavonoids and six triterpenoids were identified.This study provides an accurate and rapid method for the identification of chemical constituents of Potentilla anserina,and provides a theoretical basis for the utilization and development of potentilla anserina in the field of medicine and food.