草果中不同多酚类体外抗氧化活性研究

Study on in Vitro Antioxidant Activities of Polyphenols from Amomum tsao-ko

比较草果中两种不同多酚类体外抗氧化活性。以Vc为阳性对照,采用体外抗氧化活性方法,测定草果中不同多酚类黄酮和酚酸对DPPH·、ABTS^(+)·、·OH、O_(2)·^(-)清除能力和Fe^(3+)还原力。结果表明,草果中不同多酚类在0.625 mg/mL时对DPPH·清除率均达到80%以上,还具有一定的ABTS^(+)·、·OH、O_(2)·^(-)清除作用和Fe^(3+)还原作用。其清除效果与草果多酚浓度具有剂量依赖性。草果酚酸对DPPH·清除能力优于草果黄酮;草果黄酮对ABTS^(+)·、·OH、O_(2)·^(-)清除能力和Fe^(3+)还原能力均强于酚酸。

To evaluate in vitro antioxidant activities of two different polyphenols from Amomum tsao-ko,and to lay a foundation for the research and development of antioxidant chemical components in the A.tsao-ko.Through in vitro antioxidant activity tests,taking ascorbic acid as a positive control,the antioxidant activity of the two species of polyphenolic flavonoids and phenolic acids from A.tsao-ko on DPPH·,ABTS^(+)·,hydroxyl radical,O_(2)·^(-)and Fe^(3+)reducing ability were investigated.The scavenging rates of two different polyphenols at the concentration of 0.625 mg/mL on DPPH radical were all over 80%,they also had certain scavenging effect on ABTS^(+)radical,hydroxyl radical,superoxide anion radical and Fe^(3+)reduction.The antioxidant effects were positively correlated with the polyphenol concentration within a certain range.Comparatively,the DPPH radical scavenging ability of phenolic acids from A.tsao-ko was better than that of flavonoids,the flavonoids based on the capability to remove ABTS^(+)radical,hydroxyl radical,superoxide anion radical and Fe^(3+)reducing capacity was stronger than that of phenolic acids.