可电刺激的氧化细菌纤维素复合神经导管的制备及其表征

Preparation of Electrically Stimulable Nerve Conduit Based on Oxidized Bacterial Cellulose Composites

以吡咯为单体,三氯化铁为氧化剂,采用原位氧化聚合技术在复合了壳聚糖微球的细菌纳米纤维素(BNC)网络内部,制备了聚吡咯(Ppy)涂层。使用0.5、1.0和2.0 g/L三种不同浓度的吡咯单体,制备得到三种不同Ppy涂层的复合管(CSNPs/BNC/Ppy-1、CSNPs/BNC/Ppy-2和CSNPs/BNC/Ppy-3),从中筛选出最适宜雪旺细胞生长的Ppy浓度为2 g/L,即材料CSNPs/BNC/Ppy-3,此时接触角约为68.27°±3.43°,电导率可达到7.36×10^(-3)S/cm。再利用壳聚糖/氧化细菌纤维素复合材料(CSNPs/OBC)包封神经生长因子NGF,并通过原位氧化聚合技术获得NGF@CSNPs/OBC/Ppy复合导管,结合聚吡咯的电刺激性,通过NGF和Ppy在神经因子和支架结构上实现“双刺激法”。结果表明:该NGF@CSNPs/OBC/Ppy神经导管具备多孔微观结构,拥有可降解性和良好生物相容性,在治疗周围神经损伤方面有应用潜力。

Polypyrrole(Ppy)coating was prepared by in-situ oxidative polymerization on the chitosan microspheres/bacterial nanocellulose(BNC)composite using pyrrole as monomer.Three different Ppy coating composites(CSNPs/BNC/Ppy-1,CSNPs/BNC/Ppy-2 and CSNPs/BNC/Ppy-3)were prepared by using three different concentrations of pyrrole monomers.The most suitable concentration of Ppy for the growth of Schwann cells was 2 g/L,namely,CSNPs/BNC/Ppy-3,with a contact angle of 68.27º±3.43ºand a conductivity of 7.32×10^(-3)S/cm.Then chitosan microspheres/oxidized bacterial cellulose composite(CSNPs/OBC)was used to encapsulate nerve growth factor NGF.Combined with the electrical stimulation of polypyrrole,the“double stimulation method”was realized through NGF and Ppy based on nerve factor and scaffold structure.The results showed that the NGF@CSNPs/OBC/Ppy nerve conduit had porous microstructure,degradability and good biocompatibility,showing significant application potential in the treatment of peripheral nerve injury.