运动针对NAFLD肝脏自噬影响:动物实验Meta分析

Effects of Exercise on Liver Autophagy in NAFLD:an Animal Meta-analysis

目的通过一项动物实验Meta分析,评价运动干预是否能改善高脂饮食(HFD)诱导的大鼠或小鼠肝脏自噬水平。方法利用电子数据库中国知网(CNKI)、维普、万方、PubMed、Embase、Web of Science进行系统文献检索。严格按照纳入和排除标准采纳了18篇文献进行定量Meta分析。运用Review Manager 5.4软件对纳入文献的结局指标进行数据合并。结果Meta分析的结果显示,通过运动干预,HFD诱导的小鼠或大鼠肝脏P-AMPK/AMPK比值升高(SMD=1.43,95%CI[0.98,1.87]),LC3-Ⅱ/LC3-Ⅰ比值升高(SMD=0.44,95%CI[0.08,0.81]),LC3-Ⅱ蛋白含量升高(SMD=0.91,95%CI[0.29,1.53]),P62蛋白水平降低(SMD=-1.6,95%CI[-1.98,-1.21]),LC3蛋白总量升高(SMD=0.95,95%CI[0.4,1.51])。结论Meta分析结果表明,运动干预显著调节了由HFD诱导的动物模型肝脏中的自噬相关蛋白,使自噬水平得到了相应提升。

Objective An animal meta-analysis was conducted to evaluate whether exercise intervention can improve liver autophagy levels in rats or mice induced by high fat diet(HFD).Methods Electronic databases such as CNKI,VIP,Wanfang,PubMed,Embase and Web of Science were used for systematic literature retrieval.In strict accordance with inclusion and exclusion criteria,18 literatures were adopted for quantitative meta-analysis.Review Manager 5.4 software was used to merge the outcome indicators of the included literature.Results Meta-analysis results showed that HFD-induced liver P-AMPK/AMPK ratio increased(SMD=1.43,95%CI[0.98,1.87])and LC3-Ⅱ/LC3-Ⅰratio increased(SMD=0.44,95%CI[0.08,0.81]),LC3-Ⅱprotein content increased(SMD=0.91,95%CI[0.29,1.53]),P62 protein level decreased(SMD=-1.6,95%CI[-1.98,-1.21]),LC3 protein total increased(SMD=0.95,95%CI[0.4,1.51]).Conclusion The results of meta-analysis showed that exercise intervention significantly regulated the autophagy related proteins in the liver of animal models induced by HFD,thereby improving the level of autophagy.