摘要
为明确一株对灰霉菌具有明显抑菌效果的酵母菌菌株4-41的分类地位,明晰其抑菌防病机制,通过形态特征和26S rDNA序列分析对其进行鉴定,并利用平板对峙法检测其抗菌谱,借助灰霉菌与圣女果互作体系验证其在果实上的抑菌活性,检测其在果实伤口处的定殖动态,产挥发性抑菌物质、氨气、铁载体的能力以及溶磷、溶锌和生物成膜能力,揭示其抑菌机制。结果表明,菌株4-41为Cyberlindnera saturnus,该菌株对6种植物病原菌具有抗性,其中对灰霉菌(Botrytis cinerea)拮抗能力最强,能显著抑制灰霉菌对圣女果果实的侵染。菌株4-41能产挥发性抑菌物质,具有产铁载体和氨气的能力,能溶解培养基中的磷酸盐和氧化锌,具有生物膜形成能力,能诱导圣女果中过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)、几丁质酶(CHI)和β-1,3-葡聚糖酶(GLU)活性升高,抑制多酚氧化酶(PPO)活性。综上,拮抗酵母菌Cyberlindnera saturnus 4-41能够有效抑制灰霉菌的生长,可作为灰霉菌生物防治的有效拮抗菌材料。
In order to identify the antagonistic yeast strain 4-41,and characterize the antagonistic mechanisms,we identified the yeast strain 4-41 by the morphological characteristics and 26S rDNA sequence analysis,detected the antifungal activity by plate antagonism experiments and the interaction system between B.cinerea and cherry tomatoes,investigated the antagonistic mechanisms including the ability to produce volatile inhibitory substances,siderophores,ammonia,dissolve phosphate and zinc,and form biofilm.The results showed that the yeast strain 4-41 was identified as Cyberlindnera saturnus,which showed strong antagonism effect on six plant fungal pathogens,especially on B.cinerea,and could effectively inhibit the infection of B.cinerea on cherry tomatoes.The antagonism yeast strain 4-41 could produce volatile inhibitory substances,siderophores and ammonia,and dissolve phosphate and zinc oxide in the medium,and form biofilm.It could increase the activities of resistance related enzymes,including peroxidase(POD),phenylalanine ammonia-lyase(PAL),chitinase(CHI)andβ-1,3-glucanase(GLU),and decrease the activity of polyphenol oxidase(PPO)in cherry tomato fruit.In conclusion,the antagonism yeast strain Cyberlindnera saturnus 4-41 can effectively inhibit the growth of B.cinerea,which also can be used as an effective antagonistic material for the biological control of B.cinerea.
作者
朱露露
刘金丽
卢丹
柴春月
ZHU Lulu;LIU Jinli;LU dan;CHAI Chunyue(College of Life Science and Agricultural Engineering,Nanyang Normal University,Nanyang 473061,China)
出处
《河南农业科学》
北大核心
2023年第6期89-99,共11页
Journal of Henan Agricultural Sciences
基金
河南省高等学校重点科研项目(23A210018)
农业部华中作物有害生物综合治理重点实验室/农作物重大病虫草害防控湖北省重点实验室开放基金课题(2021ZTSJJ5)
南阳师范学院研究生创新基金项目(2022CX001)。