血清Presepsin、GRP78水平在溃疡性结肠炎诊断、病情严重程度评估中的价值

Serum Presepsin and GRP78 levels in patients with ulcerative colitis and their clinical value in diagnosis and assessment of disease activity

目的观察溃疡性结肠炎(UC)患者血清可溶性白细胞分化抗原14亚型(Presepsin)、葡萄糖调节蛋白78(GRP78)水平,探讨其对诊断、病情严重程度评估的价值。方法将2019年3月至2021年3月于该院确诊为UC并住院治疗的100例患者纳入研究作为研究组。根据UC病情严重程度将研究组患者又分为轻度组(32例)、中度组(38例)和重度组(30例)。另外,选取同期于该院体检的90例表观健康者作为对照组。采用酶联免疫法测定纳入研究者血清Presepsin、GRP78水平。分析UC患者血清Presepsin、GRP78水平与患者临床资料的关系,血清Presepsin与GRP78水平间的相关性,比较不同病情严重程度UC患者的血清Presepsin、GRP78水平。采用Logistic回归分析UC发生的影响因素。采用受试者工作特征(ROC)曲线分析血清Presepsin、GRP78水平对UC患者的诊断价值。结果研究组血清Presepsin、GRP78均高于对照组(P<0.05)。Presepsin、GRP78水平与患者年龄、性别、体重指数、饮酒史无关(P>0.05),与白细胞计数(WBC)、血小板(PLT)水平有关(P<0.05)。轻度组、中度组、重度组的UC患者血清Presepsin、GRP78水平均依次升高(P<0.05)。Pearson相关分析显示,UC患者血清Presepsin与GRP78水平呈正相关(r=0.291,P<0.05)。Logistic回归分析显示,PLT、WBC、Presepsin、GRP78均是UC发生的影响独立因素(P<0.05)。ROC曲线分析结果显示,Presepsin、GRP78单独及两者联合检测诊断UC的曲线下面积(AUC)分别为0.834、0.842、0.924,两者联合诊断的效能优于血清Presepsin、GRP78单独诊断(P<0.05)。结论UC患者血清Presepsin、GRP78均呈较高水平,其水平随病情严重程度的增加而升高,两者联合检测对UC患者具有较高的诊断效能。

Objective To observe the levels of serum Presepsin and glucose-regulated protein 78(GRP78)in patients with ulcerative colitis(UC),and to explore their clinical value in diagnosis and assessment of disease activity.Methods A total of 100 patients diagnosed with UC and hospitalized in the hospital from March 2019 to March 2021 were enrolled as the study group.According to the degree of UC disease activity,patients in the study group were further divided into mild group(32 cases),moderate group(38 cases)and severe group(30 cases).In addition,90 apparently healthy patients who underwent physical examination in this hospital during the same period were enrolled as the control group.Serum Presepsin and GRP78 levels were determined by ELISA.The relationship between serum Presepsin and GRP78 levels in UC patients and clinical data were analyzed,the correlation between serum Presepsin and GRP78 levels was analyzed,and the serum Presepsin and GRP78 levels in UC patients with different disease activity levels were compared.The influencing factors of UC were analyzed by Logistic regression.The diagnostic value of serum Presepsin and GRP78 levels in UC patients were analyzed by ROC curve.Results The serum Presepsin and GRP78 in the study group were higher than those in the control group(P<0.05).Presepsin and GRP78 levels were not correlated with age,sex,body mass index and drinking history(P>0.05),but were correlated with white blood cell count(WBC)and platelet count(PLT)(P<0.05).Serum Presepsin and GRP78 levels in mild,moderate and severe UC patients were increased successively(P<0.05).Pearson correlation analysis showed that serum Presepsin was positively correlated with GRP78 level in UC patients(r=0.291,P<0.05).Logistic regression analysis showed that PLT,WBC,Presepsin and GRP78 were all independent factors influencing the occurrence of UC(P<0.05).ROC curve analysis results showed that the area under the curve(AUC)of Presepsin and GRP78 alone and combined were 0.834,0.842 and 0.924,respectively,and the combined diagnostic efficacy was better than that of serum Presepsin and GRP78 alone(P<0.05).Conclusion Serum Presepsin and GRP78 in UC patients were at high levels,and their levels increased with the increase of disease activity.The combined detection of the two had high diagnostic efficacy in UC patients.