宫颈癌患者癌组织中lncRNA HOXA-AS2表达对患者免疫指标水平和不良生存结局的影响

Impacts of lncRNA HOXA-AS2 expression in cervical cancer tissue on the immune index levels and poor survival outcomes

目的分析宫颈癌组织中长链非编码RNA(lncRNA)HOXA簇反义RNA 2(HOXA-AS2)表达对患者免疫指标水平和预后的影响。方法选取2016年1月至2017年5月于当阳市人民医院确诊为宫颈癌的95例患者作为宫颈癌(CC)组,同期40例子宫肌瘤患者作为对照组。比较两组组织(对照组为宫颈组织,CC组为宫颈癌组织)及血清HOXA-AS2水平差异。采用Pearson相关分析HOXA-AS2与宫颈癌患者免疫指标水平的相关性。Cox回归模型分析宫颈癌患者不良生存结局的影响因素。结果与对照组比较,CC组组织及血清HOXA-AS2水平均升高,差异有统计学意义(P<0.05)。CC组患者CD8^(+)T淋巴细胞百分比、自然杀伤细胞百分比、免疫抑制酸性蛋白(IAP)水平均高于对照组,而CD3^(+)T淋巴细胞百分比、CD4^(+)/CD8^(+)比值、CD4^(+)T淋巴细胞百分比均低于对照组,差异均有统计学意义(P<0.05)。宫颈癌组织HOXA-AS2水平与IAP、CD8^(+)T淋巴细胞百分比、自然杀伤细胞百分比呈正相关(r=0.617、0.593、0.528,P<0.05),与CD3^(+)T淋巴细胞百分比、CD4^(+)/CD8^(+)比值、CD4^(+)T淋巴细胞百分比呈负相关(r=-0.506、-0.539、-0.482,P<0.05)。分化程度为低分化、发生淋巴结转移、FIGO分期为Ⅲ~Ⅳ期及HOXA-AS2高表达是宫颈癌患者不良生存结局的危险因素(P<0.05)。结论HOXA-AS2在宫颈癌中表达水平升高,其与宫颈癌患者免疫指标水平密切相关,是宫颈癌患者不良生存结局的危险因素。

Objective To analyze the influences of long non-coding RNA(lncRNA)HOXA cluster antisense RNA 2(HOXA-AS2)expression in cervical cancer tissue on the level of immune indexes and prognosis of patients.Methods A total of 95 patients diagnosed with cervical cancer in Dangyang People′s Hospital from January 2016 to May 2017 were enrolled as the cervical cancer(CC)group,and 40 patients with uterine fibroids during the same period were enrolled as the control group.The expression levels of HOXA-AS2 in tissue(cervical tissue in control group,cervical cancer tissue in CC group)and serum were compared between the two groups.The correlation between HOXA-AS2 and immune indexes was analyzed by using Pearson correlation.The Cox regression model was used to explore the influencing factors of poor survival outcomes in patients with cervical cancer.Results Compared with the control group,the expression levels of HOXA-AS2 in the tissue and serum of the CC group were increased,and the difference was statistically significant(P<0.05).The percentage of CD8^(+)T lymphocytes,natural killer cells and level of immunosuppressive acidic protein(IAP)in the CC group were higher than those in the control group,while percentage of CD3^(+)T lymphocytes,CD4^(+)/CD8^(+)ratio,and percentage of CD4^(+)T lymphocytes were lower than those in the control group,and the differences were statistically significant(P<0.05).The expression level of HOXA-AS2 in cancer tissue was positively correlated with IAP level,percentage of CD8^(+)T lymphocytes and natural killer cells(r=0.617,0.593,0.528,P<0.05),and was negatively correlated with percentage of CD3^(+)T lymphocytes,CD4^(+)/CD8^(+)ratio and percentage of CD4^(+)T lymphocytes(r=-0.506,-0.539,-0.482,P<0.05).Low differentiation,lymph node metastasis,FIGO stageⅢ-Ⅳand high expression of HOXA-AS2 were risk factors for poor survival outcomes in patients with cervical cancer(P<0.05).Conclusion The expression level of HOXA-AS2 is increased in cervical cancer,which is closely related to the level of immune indicators,and is the risk factors of the poor survival outcome of cervical cancer patients.