绿茶衍生碳点用于光动力治疗耐药菌感染

Thea Viridis Derived Carbon Dots for Drug-resistant Bacterial Infections by Photodynamic Therapy

随着细菌耐药性的不断播散,尤其是“超级细菌”的出现,临床可用抗生素药物越来越少,迫切需要发展新的高效、低毒和无耐药性的抗菌材料和技术。本研究采用生物质绿茶作为碳源,通过溶剂热法,成功制备了具有光动力治疗(Photodynamic therapy,PDT)性能的绿茶衍生碳点(T‐CDs)。在660 nm激光照射下,所制备的T‐CDs能够有效产生活性氧。细胞和活体实验表明,T‐CDs具有优异的生物相容性,且产生的活性氧能杀死耐甲氧西林金黄色葡萄球菌,从而通过降低细菌引起的伤口炎症,加速伤口愈合。本研究所制备的T‐CDs能够通过PDT杀灭致病菌,促进感染伤口愈合,为开发抗生素替代药物提供了新的思路,同时对探索耐药菌感染伤口临床治疗新方案具有重要参考价值。

With the spread of bacterial drug resistance,especially the emergence of“superbugs”,it's an urgent need to develop new antibacterial materials and technologies with high efficiency,low toxicity and no drug resistance.In this study,thea viridis derived carbon dots(T-CDs)were successfully prepared by solvothermal method.Under 660 nm laser irradiation,the prepared T-CDs could effectively produce reactive oxygen species(ROS).In vi⁃tro and in vivo experiments showed that T-CDs have excellent biocompatibility,and can produce ROS under laser irradiation to kill methicillin-resistant Staphylococcus aureus,thereby reducing wound inflammation caused by bacteria and accelerating wound healing.The prepared T-CDs can kill pathogenic bacteria through PDT and promote the healing of infected wounds,provide a new idea for the development of antibiotic replacement drugs,and have important value for exploring new clinical treatment schemes of drug-resistant bacteria infected wounds.