制貂肾中黄曲霉毒素的测定方法研究及暴露风险评估

Determination Method of Aflatoxin in Processed Mustela and Risk Assessment

目的:本研究基于免疫亲和柱净化样品,采用高效液相色谱法光化学衍生荧光检测器测定制貂肾中黄曲霉毒素B_(1)、B_(2)、G_(1)、G_(2)的含量并对其进行暴露风险评估。方法:样品采用70%甲醇作为提取溶剂,经免疫亲和柱净化、高效液相色谱分离、光化学柱后衍生后,通过荧光检测器测定其中黄曲霉毒素的含量。采用暴露边界比对制貂肾黄曲霉毒素的有害残留物进行风险评估。结果:结果表明黄曲霉毒素B_(1)的线性范围为0.0104~0.0520 ng(r=0.9999)、黄曲霉毒素B_(2)的线性范围为0.0038~0.0190 ng(r=0.9998)、黄曲霉毒素G_(1)的线性范围为0.0108~0.0540 ng(r=0.9998)、黄曲霉毒素G_(2)的线性范围为0.0038~0.0190 ng(r=0.9998),线性关系良好,回收率在89.31%~99.54%间,RSD≤3.1%。结论:建立的制貂肾中黄曲霉毒素检测方法具有操作简单,灵敏度高、重复性好、结果准确,可用于制貂肾中黄曲霉毒素的测定,暴露风险评估制貂肾的黄曲霉毒素对人体健康风险较低。

Objective:In this study,based on immunoaffinity column purification sample,the content of aflatoxin B_(1),B_(2),G_(1),G_(2)in Processed Mustela was simultaneously determined by photochemical derivatization HPLC-fluorescence detection,and the risk was evaluated.Methods:The sample was extracted with 70%methanol,purified by immunoaffinity column,separated by HPLC,and derivatized by photochemical column.The content of aflatoxin in the sample was determined by fluorescence detector.Risk assessment of harmful residues of aflatoxin in mink kidney was carried out by exposure boundary ratio.Results:The results showed that the linear range of aflatoxin B1 was 0.0104-0.0520 ng(r=0.9999),the linear range of aflatoxin B2 was 0.0038-0.0190 ng(r=0.9998),the linear range of aflatoxin G1 was 0.0108-0.0540 ng(r=0.9998),and the linear range of aflatoxin G2 was 0.0038-0.0190 ng(r=0.9998).The linear relationship was good,the recovery was 89.31%-99.54%,and the RSD was≤3.1%.Conclusion:The established detection method is simple,sensitive,reproducible and accurate.It can be used for the determination of aflatoxin in mink kidney.The risk assessment of aflatoxin in mink kidney has low risk to human health.