决明苯丙氨酸解氨酶(PAL)基因家族的全基因组分析

Genome wide analysis of the phenylalanine ammonia lyase(PAL)gene family from Senna tora

利用HMMER、Pfam与SMART等工具,从决明(Senna tora)等不同物种中筛选获得26条PAL(苯丙氨酸解氨酶)序列,并对其进行生物信息学分析。结果表明,决明PAL基因家族的基本特征在单双子叶植物分离之前就已形成,且6个PAL成员被分为3个亚类。决明PAL基因家族成员的二级结构主要由α-螺旋和无规则卷曲组成;含有较高的Ala、Ser和Leu残基;存在16种不同的保守基序,其中motif12(含有催化关键序列Ala-Ser-Gly)在所有PAL成员中均出现;启动子区含有较多的光反应、植物激素响应与逆境胁迫响应元件。进一步的GO、转录组与Pearson分析也强化了以上分析结果,即决明PAL基因家族成员多集中在叶、茎和花等易感受光的组织,参与抗旱与抗菌等生物学过程呈现多样化的特点。以上分析结果将为决明PAL基因的后续功能研究及抗逆功能基因筛选提供理论基础。

Using tools such as HMMER,Pfam,and SMART,26 PAL(phenylalanine ammonia lyase)sequences were screened from different species such as Senna tora,and analyzed for bioinformatics.The results showed that the basic characteristics of the PAL gene family of Senna tora had been formed before the isolation of monocotyledonous and dicotyledonous plants,and the six PAL members were divided into three subclasses.The secondary structure of the members of the PAL gene family of Senna tora was mainly composed ofα-helixes and irregular curls,containing higher Ala,Ser,and Leu residues;there were 16 different conservative motifs,among which motif12(containing the catalytic key sequence Ala Ser Gly)appeared in all PAL members;the promoter region contained many elements for light response,plant hormone response,and stress response.Further GO,transcriptome,and Pearson analyses had also reinforced the above analysis results,indicating that members of the PAL gene family of Senna tora were mostly concentrated in lightsensitive tissues such as leaves,stems,and flowers,and participated in diverse biological processes such as drought resistance and antibacterial activities.The above analysis results would provide a theoretical basis for the subsequent functional study of the PAL gene of Senna tora and the screening of stress resistant functional genes.