鸡HSP70家族基因鉴定及功能分析

Identification and functional analysis of the chicken HSP70 family genes

热激蛋白70(HSP70)是一类广泛存在于动植物体内的分子伴侣,并在各类生物或非生物胁迫过程中发挥重要作用。目前多种动植物体内的HSP70已经得到系统鉴定,但对于鸡HSP70尚缺乏全面分析,鉴于此,本研究采用生物信息学方法对鸡基因组进行挖掘、鉴定和分析。结果表明,在鸡基因组中鉴定出17个HSP70基因家族成员,它们分散在7条染色体上,出现6次串联重复,且无共线性。HSP70家族蛋白质理化性质差异较大,氨基酸序列长度为472~998 aa,包含15个酸性蛋白质和2个碱性蛋白质,仅HSPA13(rna-NM_001030793.4)出现1次跨膜。同一进化分支上,各成员的保守结构域具有一定相似性,且所有成员均具有Motif1。进化树分析结果表明,鸡、人、鼠3个物种HSP70中相同亚家族成员聚集在一起,表明HSP70家族在不同物种间具有一定的保守性。新城疫病毒感染鸡的转录组数据分析结果表明,在新城疫病毒感染过程中,多数HSP70基因家族成员表达量发生不同程度下调,其中HSPA8(rna-NM_205003.3)和HSPA2(rna-NM_001006685.1)表达量下调明显,二者有望成为新城疫防治的新靶点。本研究结果不仅为鸡HSP70生物学功能研究奠定基础,还为其他物种相关研究提供理论参考。

Heat shock protein 70(HSP70)is a kind of molecular chaperone widely found in animals and plants,and plays an important role in various biotic or abiotic stress processes.At present,HSP70 has been systematically identified in various plants and animals,but there is still a lack of comprehensive analysis of chicken HSP70.In view of this,this study adopted bioinformatics methods to excavate,identify and analyze the chicken genome.The results showed that a total of 17 HSP70 gene family members were identified in the chicken genome,which were scattered on seven chromosomes,with six tandem duplications and no collinearity.The physical and chemical properties of HSP70 family proteins were quite different.The amino acid sequence length was between 472 aa and 998 aa,and there were 15 acidic proteins and two basic proteins.HSPA13(rna-NM_001030793.4)was the only transmembrane protein.The conserved domains of members on the same evolutionary branch were similar to each other,and all members had Motif1.The results of phylogenetic tree analysis showed that the same subfamily members of HSP70 in chicken,human and mouse were clustered together,indicating that HSP70 family was conservative among different species.The results of transcriptome data analysis of chickens infected with Newcastle disease virus showed that the expression levels of most members were down-regulated in different degrees during the infection of Newcastle disease virus.The expression levels of HSPA8(rna-NM_205003.3)and HSPA2(rna-NM_001006685.1)were significantly down-regulated,and they were expected to become new targets for Newcastle disease prevention and treatment.The results of this study not only lay a foundation for the study of the biological function of chicken HSP70,but also provide a theoretical reference for the related research of other species.