生物正交点击化学在生物成像中的应用

Application of bioorthogonal click chemistry in bioimaging

近年来,随着生物正交点击化学的快速发展,在生命体系中实现特异性选择标记和成像生物分子成为了可能。生物正交点击化学反应能够在保证对生物活性干扰忽略不计的前提下,在生物系统中实现两个外源互补部分之间快速和选择性化学反应的能力,这使得其在多种生物应用中具有很高的前景。该文综述了生物正交点击化学在生物成像中的最新进展。该文讨论了在体内系统中常见的生物正交点击反应及其在体内的生物标记和成像应用,特别是在细胞中生物分子、肿瘤和细菌的可视化方面。并对生物正交领域的潜在发展方向进行了展望。

In recent years,with the rapid development of bioorthogonal click chemistry,it has become possible to achieve specific selection markers and imaging biomolecules in living systems.The ability of bioorthogonal click chemistry reactions to achieve rapid and selective chemical reactions between two exogenous complementary moieties in biological systems with negligible interference with biological activity makes them useful in a variety of biological applications.Has high prospects.This article reviews the recent progress of bioorthogonal click chemistry in bioimaging.This article discusses common bioorthogonal click reactions in in vivo systems and their in vivo biomarker and imaging applications,especially in the visualization of biomolecular processes,therapeutic cells,tumors,and bacteria.And the potential development direction of the bioorthogonal field is prospected.