胃癌肿瘤微环境中预后相关基因研究

Prognostic related genes in tumor microenvironment of gastric cancer

目的探讨胃癌肿瘤微环境中差异表达基因与胃癌预后的关系。方法基于TCGA数据库中胃癌基因数据,筛选与基质细胞和免疫细胞相关的差异表达基因,对差异表达基因进行基因本体论富集分析和蛋白质-蛋白质相互作用网络分析,分析差异表达基因与胃癌预后的关系。结果不同病理分级胃癌患者基质评分、免疫评分及综合评分比较,差异有统计学意义(P<0.05)。G3级患者免疫评分最高,G1级患者略高,G2级患者最低,其中G1级患者与G2级患者免疫评分比较,差异无统计学意义(P>0.05)。基质评分随着肿瘤级别的增高而增高。分化良好的G1级患者综合评分最低,分化较低的G2级患者次之,恶性程度最高的G3级患者最高,其中G1级患者与G2级患者综合评分比较,差异无统计学意义(P>0.05)。上调表达基因分别与基质评分、免疫评分具有显著相关性(P<0.05)。CXCR4(P=0.002)、NPY1R(P=0.002)、BPI(P=0.019)、MCEMP1(P=0.009)、OLR1(P=0.041)、RETN(P=0.045)表达水平越高,胃癌患者生存时间越短。结论差异表达基因在胃癌肿瘤免疫中发挥着不可或缺的重要作用,其中BPI、CXCR4、MCEMP1、NPY1R、OLR1、RETN基因高表达是胃癌预后的不良因素。

Objective To explore the relationship between differentially expressed genes in tumor microenvironment and prognosis of gastric cancer.Methods Based on the gastric cancer gene data in the TCGA database,the differentially expressed genes related to stromal cells and immune cells were screened.The differentially expressed genes were analyzed by Gene Ontology enrichment analysis and protein-protein interaction network analysis,and the relationship between differentially expressed genes and the prognosis of gastric cancer was analyzed.Results There were statistically significant differences in the stromal score,immune score,and comprehensive score among gastric cancer patients with different pathological grades(P<0.05).G3 grade patients had the highest immune score,G1 grade patients had a slightly higher score,and G2 grade patients had the lowest score.There was no statistically significant difference in the immune scores between G1 grade patients and G2 grade patients(P>0.05).The stromal score increased with the increase of tumor grade.G1 grade patients with good differentiation had the lowest comprehensive score,followed by G2 grade patients with lower differentiation,and G3 grade patients with the highest degree of malignancy had the highest comprehensive score.There was no statistically significant difference in the comprehensive score between G1 grade patients and G2 grade patients(P>0.05).The upregulated expression of genes was significantly correlated with the stromal score and immune score,respectively(P<0.05).The expression level of CXCR4(P=0.002),NPY1R(P=0.002),BPI(P=0.019),MCEMP1(P=0.009),OLR1(P=0.041),RETN(P=0.045)was the higher,the survival time of gastric cancer patients was the shorter.Conclusion Differentially expressed genes play an indispensable and important role in the immune response of gastric cancer tumors,with high expression of BPI,CXCR4,MCEMP1,NPY1R,OLR1,and RETN genes being adverse factors for the prognosis of gastric cancer.