苦荞抗立枯病基因FtABCG12的克隆及其功能分析

Molecular Cloning and Functional Analysis of Resistance Gene FtABCG12 of Tartary Buckwheat to Blight

以川荞1号为材料,克隆得到FtABCG12基因。基因结构分析表明,FtABCG12基因由7个外显子和6个内含子组成,cDNA全长1734bp,编码577个氨基酸。系统进化树显示,FtABCG12蛋白与拟南芥AtABCG11、AtABCG12、AtABCG13和AtABCG15蛋白亲缘关系较近。qRT-PCR结果表明,FtABCG12基因在苦荞中的表达受立枯丝核菌侵染的诱导。转基因拟南芥中,过表达FtABCG12基因可显著提高拟南芥植株的抗病性。以上结果证明,FtABCG12基因参与苦荞生物胁迫的适应过程,为进一步研究FtABCG12调控苦荞抗立枯病的分子机制奠定了基础。

FtABCG12 gene was cloned from tartary buckwheat Chuanqiao No.1.The FtABCG12 gene was composed of seven exons and six introns with a full length cDNA of 1734bp,encoding 577 amino acids.Phylogenetic tree showed that FtABCG12 protein was closely related to Arabidopsis thaliana AtABCG11,AtABCG12,AtABCG13 and AtABCG15 proteins.qRT-PCR results indicated that the expression of FtABCG12 gene in tartary buckwheat was induced by the infection of Rhizoctonia solani.In transgenic A.thaliana,overexpression of FtABCG12 gene significantly increased the disease resistance of A.thaliana plants.These results proved that FtABCG12 was involved in the adaptation process of tartary buckwheat under biological stress,which laid the foundation for further study on the molecular mechanism of FtABCG12 regulation of tartary buckwheat resistance to blight.