四川凉山苦荞种子携带真菌多样性分析

Analysis of Fungal Diversity in Seeds of Tartary Buckwheat from Liangshan,Sichuan Province

苦荞是四川省凉山彝族自治州主要的粮食作物之一,病原真菌引起的叶部和根部病害严重影响了苦荞的产量与品质,种子带菌是引起病害发生的重要初侵染来源。对来自凉山州7个县的46份苦荞种子的内、外部携带真菌类群进行了分离培养,及基于18S r RNA基因扩增子测序的检测。结果表明,分离培养法从种子内部和外部分别获得786和594株真菌分离物,共17个属,其中镰刀菌属(Fusarium)和链格孢属(Alternaria)分别是种子内部寄藏和外部携带的优势菌群。18S rRNA基因扩增子测序法分别从种子内部和外部获得39个和41个属的真菌,核盘菌属(Sclerotinia)同时为苦荞种子内部寄藏与外部携带的优势菌群。同一地区不同样品的菌群多样性存在差异。

Tartary buckwheat(Fagopyrum tataricum)is one of the staple food crops in Liangshan Yi Autonomous Prefecture,Sichuan province.The leaf and root diseases caused by pathogenic fungi seriously affected the yield and quality of tartary buckwheat.Pathogen in seeds are important primary infection source of disease.In this study,46 samples of tartary buckwheat seed from seven counties of Liangshan Prefecture were studied by agar plating method and 18S rRNA amplicon sequencing method.The results showed that a total of 786 fungal isolates and 594 fungal isolates were obtained from inside and outside of seeds by agar plating,respectively,which belonged to 17 genera.Among them,Fusarium and Alternaria were the dominant seed-borne microflora for the inside and outside of the seeds,respectively.A total of 39 genera and 41 genera were identified from inside and outside of seeds by 18S rRNA amplicon sequencing method,respectively.Sclerotinia was the dominant seed-borne microflora for the inside and outside of the tartary buckwheat seeds.Moreover,it was found that different samples from the same area had fungal diversity differences.