摘要
目的:构建一种新型的高灵敏度的电化学传感器对苦荞中的芦丁进行定量分析。方法:在四氧化三铁(Fe_(3)O_(4))纳米材料表面负载一层二氧化钛(TiO_(2))制备核壳结构的Fe_(3)O_(4)@TiO_(2)纳米复合材料,在其表面负载纳米金(Au)制备了一种新型的Fe_(3)O_(4)@TiO_(2)@Au纳米复合材料,并构建了电化学传感器对芦丁进行定量分析。通过对纳米材料的电化学、芦丁的电化学表征、缓冲液pH、富集时间、富集电位等条件的优化确定传感器的最佳工作条件。结果:Fe_(3)O_(4)@TiO_(2)@Au具有良好的吸附性、导电性,可有效提高传感器的灵敏度。芦丁浓度与其对应的氧化峰电流分别在0.02~20.00,20.00~200.00μmol/L的浓度范围内呈线性关系,检出限(S/N=3)为0.006 4μmol/L,线性范围较宽、检出限较低,明显优于其他电化学传感器且制备的传感器重复性、稳定性较好,对常见共存物有很好的抗干扰能力。结论:制备的传感器操作简单、成本低且纳米复合材料可回收再利用。
Objective:A novel electrochemical sensor with high sensitivity was developed for the quantitative analysis of rutin in tartary buckwheat.Methods:A layer of titanium dioxide was loaded on the surface of ferric oxide nano materials(Fe_(3)O_(4)) to prepare core-shell Fe_(3)O_(4)@titanium dioxide(Fe_(3)O_(4)@TiO_(2)) nano composites,and nano gold(Au) was loaded on its surface for preparing a new type of Fe_(3)O_(4)@TiO_(2)@Au nanocomposites.Then an electrochemical sensor was constructed for quantitative analysis of rutin.The optimal working conditions of the sensor were determined by electrochemical characterizing of nano materials,rutin and optimizing,buffer pH,enrichment time,enrichment potential and other conditions.Results:Fe_(3)O_(4)@TiO_(2)@Au had good adsorption and conductivity,which could effectively improve the sensitivity of the sensor.Rutin concentration and its corresponding oxidation peak current show linear relationship in the concentration range of 0.02~20 μmol/L and 20~200 μmol/L,respectively.The detection limit(S/N=3) was 0.006 4 μmol/L,and the linear range was wide and detection limit was low.It is obviously better than other electrochemical sensors,and the sensor prepared had good repeatability,stability and good anti-interference ability to common co-existing objects.Conclusion:The prepared sensor had the advantages of simple operation,low cost and the potential of recycling of nanocomposites,which provides a more effective method for rapid quantitative analysis of rutin.
作者
周鸿燕
宋鋆
ZHOU Hong-yan;SONG Yun(Jiyuan Vocational and Technical School,Jiyuan,Henan 459000,China)
出处
《食品与机械》
CSCD
北大核心
2023年第6期81-87,共7页
Food and Machinery
基金
河南省科学技术厅科研项目(编号:192102210237)。
