杉木组培苗增殖光环境优化

Optimization of the light environment for the propagation of tissue⁃cultured Cunninghamia lanceolata seedlings

为获得杉木组培苗增殖的最佳光照条件,采用3因素5水平的二次正交旋转组合设计对其增殖系数、生长量和光合特性参量等进行研究。结果表明:光质对杉木组培苗不定芽增殖系数和叶片光系统Ⅱ(PSⅡ)的最大光化学效率(F_(v)/F_(m))的作用大小均为蓝光>红光>绿光,蓝光和红光对增殖系数的影响显著(P<0.05),蓝光对F_(v)/F_(m)的影响显著(P<0.05);增殖系数随各光质的光强增大而增大,F_(v)/F_(m)随蓝光和红光光强增大而增大、随绿光光强增大而减小;红光和蓝光、红光和绿光的交互作用分别对增殖系数和F_(v)/F_(m)的影响达极显著(P<0.01)和显著(P<0.05)水平;增殖的最优光质光强组合为开放科学标识码(OSID码)红光20.0μmol·m^(-2)·s^(-1)、绿光2.1μmol·m^(-2)·s^(-1)、蓝光30.0μmol·m^(-2)·s^(-1),可获得最大增殖系数(3.57);Fv/Fm的最优光质光强组合为红光20.0μmol·m^(-2)·s^(-1)、绿光2.0μmol·m^(-2)·s^(-1)、蓝光23.8μmol·m^(-2)·s^(-1),可获得最大Fv/Fm值(0.78)。光质主效应对鲜重的作用大小为绿光>红光>蓝光,红光对鲜重的影响显著(P<0.05),鲜重的最优光质光强组合为红光28.0μmol·m^(-2)·s^(-1)、绿光2.0μmol·m^(-2)·s^(-1)、蓝光30.0μmol·m^(-2)·s^(-1),可获得最大鲜重(0.21 g)。此外,各光质光强组合对株高和叶面积的影响差异极显著(P<0.01),但对叶绿素(a+b)含量的影响差异不显著。红光有利于提高杉木组培苗生物量,蓝光有利于提高杉木组培苗的光合能力,红光和蓝光有利于提高杉木组培苗不定芽的增殖系数;光质对杉木组培苗不定芽的增殖系数和F_(v)/F_(m)的影响具有一定的相关性,暗示光质通过调控光合作用进而调控杉木不定芽的增殖能力。

To obtain optimum light conditions for the proliferation of tissue⁃cultured Cunninghamia lanceolata seedlings,the proliferation coefficient,growth,and photosynthetic parameters were studied using a quadratic orthogonal rotation combination design with three factors and five levels.The results showed that light quality was correlated to the proliferation coefficient of adventitious buds of tissue⁃cultured C.lanceolata seedlings,and the maximum photochemical efficiency(F_(v)/F_(m))of photosystemⅡ(PSⅡ)of leaves was in the order of blue light>red light>green light;red and blue light had a significant effect on the proliferation coefficient(P<0.05)and blue light had a significant effect on F_(v)/F_(m)(P<0.05).The proliferation coefficient increased with the light intensities of each light quality;F_(v)/F_(m)increased with an increase in blue and red light intensities,whereas it decreased with an increase in green light intensity.The interactions between red and blue light and red and green light had significant effects on the proliferation coefficient(P<0.01)and F_(v)/F_(m)(P<0.05),respectively.An optimal light intensity combination of red light 20.0μmol·m^(-2)·s^(-1),green light 2.1μmol·m^(-2)·s^(-1),blue light 30.0μmol·m^(-2)·s^(-1)resulted in a maximum proliferation coefficient of 3.57,and a combination of red light 20.0μmol·m^(-2)·s^(-1),green light 2.0μmol·m^(-2)·s^(-1),blue light 23.8μmol·m^(-2)·s^(-1)resulted in a maximum Fv/Fm of 0.78.The effect of light quality on the fresh weight of the seedlings was green light>red light>blue light,and the effect of red light on fresh weight was significant(P<0.05).A light intensity combination of red light 28.0μmol·m^(-2)·s^(-1),green light 2.0μmol·m^(-2)·s^(-1),blue light 30.0μmol·m^(-2)·s^(-1)resulted in the maximum fresh weight of 0.21 g.In addition,the effects of different light intensity combinations on plant height and leaf area were significant(P<0.01);however,chlorophyll(a+b)content was not significantly affected.The red light was conducive to increasing biomass,the blue light was conducive to improving photosynthetic capacity,and the red and blue lights were beneficial in improving the proliferation coefficient of adventitious buds of tissue⁃cultured C.lanceolata seedlings.The light quality was correlated to the proliferation coefficient and Fv/Fm of adventitious buds,suggesting that light quality regulates proliferation by regulating the photosynthesis of adventitious buds.