AM真菌与印度梨形孢对干旱胁迫下垂丝海棠生长发育和水分利用的影响

Effects of arbuscular mycorrhizal fungi and Piriformaospora indica on growth and water use of Malus halliana under drought stress

采用盆栽土培试验,研究丛枝菌根真菌处理(AM)、印度梨形孢真菌(PI)及其复合接种处理(AM+PI)对正常水分(WW)和干旱胁迫(DS)下垂丝海棠生长、生理特性及水分利用的影响。结果表明:无论正常水分还是干旱胁迫条件下,与不施菌剂处理相比,丛枝菌根真菌处理、印度梨形孢真菌处理和丛枝菌根真菌+印度梨形孢真菌处理均在一定程度上促进了垂丝海棠的生长,改善了植株光合生理,激活了抗氧化系统,提高了植株水分利用效率;丛枝菌根真菌处理在缓解干旱胁迫相关的生长发育及水分利用方面更为有效,而印度梨形孢真菌在促进内部生理代谢方面表现更佳。整体而言,干旱条件下丛枝菌根真菌+印度梨形孢真菌处理效果最为显著,叶片相对含水量比正常水分条件下对照提高了4.41%,水分利用效率提高了26.92%。综上,在干旱环境中接种丛枝菌根真菌、印度梨形孢真菌均可有效促进植物生长发育及相关生理代谢,前者主要通过表型改善缓解干旱胁迫,后者主要通过调节内在生理代谢实现,以复合接种效果最佳。

A pot soil culture experiment was used to study the effects of arbuscular mycorrhizal fungi(AM),Piriformaospora indica(PI)and their combined treatment(AM+PI)on the growth,physiological characteristics and water use fo Malus halliana under normal water(WW)and drought stress(DS).The results showed that under WW or DS conditions,AM,PI and AM+PI treatment promoted the growth of Malus halliana to a certain extent,and improved the photosynthetic physiology of plants,compared with the control treatment(CK).Activated the antioxidant system and improved plant water use efficiency;AM was more effective in alleviating drought stress-related growth and water use,and PI was better in promoting internal physiological metabolism.Overall,the combined treatment(AM+PI)had the most significant effect under drought conditions.The relative water content and water use efficiency of leaves in AM+PI treatment were 4.41%and 26.92%higher than those in WW-CK treatment,respectively.In conclusion,inoculation with arbuscular mycorrhizal fungi and Piriformaospora indica in arid environment can effectively promote plant growth and development and related physiological metabolism.The former mainly alleviates drought stress through phenotypic improvement,while the latter mainly achieves by regulating internal physiological metabolism,and combine treatment the best inoculation effect.