糖基化基因B4GALT5在胰腺癌恶性进展中的作用

Role of glycosylation gene B4GALT5 in malignant progression of pancreatic cancer

目的:基于糖基化基因集在TCGA⁃GTEx联合公共数据库中挖掘与胰腺癌发生风险和不良预后显著相关的基因,利用本中心的组织样本和体外实验进行验证。方法:通过公共数据库筛选到与胰腺癌发生风险和不良预后相关的糖基化基因B4GALT5,使用本中心有临床与预后信息的组织样本进行验证。构建B4GALT5干扰和过表达的胰腺癌细胞系,通过CCK⁃8、细胞划痕和Transwell侵袭实验观察B4GALT5在体外对胰腺癌细胞恶性生物学行为的作用。结果:胰腺癌组织中B4GALT5的表达显著高于正常组织,并且B4GALT5的表达量增高与胰腺癌发生风险和不良预后呈显著正相关,与CD4+T细胞和自然杀伤T细胞浸润呈负相关。B4GALT5在胰腺癌细胞的体外增殖、运动、浸润过程中起促进作用。结论:糖基化基因B4GALT5有促进胰腺癌恶性进展的作用,可能作为新的生物标志物预测胰腺癌发生风险与不良预后。

Objective:Based on the glycosylation related gene set,the TCGA⁃GTEx joint public database was used to identify genes significantly related to the occurrence and poor prognosis of pancreatic cancer,and the tissue samples of our center and in vitro experiments were used for verification.Methods:The glycosylation gene B4GALT5 related to the risk and poor prognosis of pancreatic cancer was screened through the public database,and was verified using the tissue samples of our center with clinical and prognostic information.We constructed pancreatic cancer cell lines with B4GALT5 interference and overexpression,and observed the effects of B4GALT5 on the malignant biological behavior of pancreatic cancer cells in vitro through CCK-8,wound healing,transwell invasion experiments.Results:The expression of B4GALT5 in pancreatic cancer tissue was significantly higher than that in normal pancreatic tissue.The increased expression of B4GALT5 was positively correlated with the risk and poor prognosis of pancreatic cancer,and negatively correlated with the infiltration of CD4+T cells and NK T cells.B4GALT5 played a role in exacerbating the proliferation,movement and invasion of pancreatic cancer cells in vitro.Conclusion:The glycosylation gene B4GALT5 can promote the malignant progression of pancreatic cancer,and may be used as a new biomarker to indicate the risk and poor prognosis of pancreatic cancer.