摘要
为了获得适用于治疗骨髓炎骨组织缺损及修复的生物材料,受贻贝蛋白的强黏附性能的启发,以掺铜的生物玻璃(Cu-MBG)纳米颗粒为基底,在Cu-MBG表面涂覆聚多巴胺(PDA),得到Cu-MBG@PDA;对PDA涂覆前后的Cu-MBG纳米颗粒的形貌和结构进行表征,分析其亲水性、体外生物活性及抗菌性能。结果表明:经过PDA涂覆的Cu-MBG@PDA的水接触角减小到44.31°,亲水性能明显提高;在体外模拟体液(SBF)中浸泡之后,Cu-MBG@PDA样品比Cu-MBG有着更快的羟基磷灰石形成能力;经过PDA涂覆的Cu-MBG@PDA具有优异的抗菌性能,对金黄色葡萄球菌(Staphylococcusaureus,S.aureus)和大肠杆菌(Escherichiacoli,E.coli)的抑制率都提高到99.90%以上。经过PDA涂覆的Cu-MBG@PDA具有更优异的亲水性、体外生物活性及抗菌性能,有望应用于慢性骨髓炎导致的骨组织缺损及修复方面的治疗。
To obtain biomaterials suitable for the treatment of bone tissue defects and repair in osteomyelitis,we,inspired by the strong adhesive properties of mussel proteins,obtained Cu-MBG@PDA by coating polydopamine(PDA)on the surface of Cu-MBG using copper-doped bioglass(Cu-MBG)nanoparticles as the substrate.We characterized the morphology and structure of Cu-MBG nanoparticles before and after PDA coating to analyze their hydrophilicity,in vitro bioactivity and antibacterial properties.The results show that the water contact angle of Cu-MBG@PDA coated with PDA is reduced to 44.31°,and the hydrophilic performance is significantly improved.The ability of Cu-MBG@PDA samples to form hydroxyapatite is faster than Cu-MBG after immersion in in vitro simulated body fluids(SBF).The inhibitory rates of PDA-coated Cu-MBG@PDA against Staphylococcus aureus and Escherichia coli both increase to over 99.90%,with excellent antibacterial properties.PDA-coated Cu-MBG@PDA has superior hydrophily,in vitro bioactive and antibacterial properties,and is expected to be used in the treatment of bone tissue defects and repair caused by chronic osteomyelitis.
作者
贺蓉
刘涛
丁新波
仇巧华
朱灵奇
HE Rong;LIU Tao;DING Xinbo;QIU Qiaohua;ZHU Lingqi(College of Textile Science and Engineering(International Institute of Silk),Zhejiang Sci-Tech University,Hangzhou 310018,China)
出处
《浙江理工大学学报(自然科学版)》
2023年第4期508-515,共8页
Journal of Zhejiang Sci-Tech University(Natural Sciences)
基金
国家自然科学基金项目(31900964)。
关键词
生物活性玻璃
铜
聚多巴胺
生物活性
抗菌性能
bioactive glass
copper
polydopamine
biological activity
antibacterial properties