聚多巴胺涂覆Cu-MBG的生物活性及抗菌性能研究

Study on the biological activity and antibacterial properties of polydopamine-modified Cu-MBG

为了获得适用于治疗骨髓炎骨组织缺损及修复的生物材料,受贻贝蛋白的强黏附性能的启发,以掺铜的生物玻璃(Cu-MBG)纳米颗粒为基底,在Cu-MBG表面涂覆聚多巴胺(PDA),得到Cu-MBG@PDA;对PDA涂覆前后的Cu-MBG纳米颗粒的形貌和结构进行表征,分析其亲水性、体外生物活性及抗菌性能。结果表明:经过PDA涂覆的Cu-MBG@PDA的水接触角减小到44.31°,亲水性能明显提高;在体外模拟体液(SBF)中浸泡之后,Cu-MBG@PDA样品比Cu-MBG有着更快的羟基磷灰石形成能力;经过PDA涂覆的Cu-MBG@PDA具有优异的抗菌性能,对金黄色葡萄球菌(Staphylococcusaureus,S.aureus)和大肠杆菌(Escherichiacoli,E.coli)的抑制率都提高到99.90%以上。经过PDA涂覆的Cu-MBG@PDA具有更优异的亲水性、体外生物活性及抗菌性能,有望应用于慢性骨髓炎导致的骨组织缺损及修复方面的治疗。

To obtain biomaterials suitable for the treatment of bone tissue defects and repair in osteomyelitis,we,inspired by the strong adhesive properties of mussel proteins,obtained Cu-MBG@PDA by coating polydopamine(PDA)on the surface of Cu-MBG using copper-doped bioglass(Cu-MBG)nanoparticles as the substrate.We characterized the morphology and structure of Cu-MBG nanoparticles before and after PDA coating to analyze their hydrophilicity,in vitro bioactivity and antibacterial properties.The results show that the water contact angle of Cu-MBG@PDA coated with PDA is reduced to 44.31°,and the hydrophilic performance is significantly improved.The ability of Cu-MBG@PDA samples to form hydroxyapatite is faster than Cu-MBG after immersion in in vitro simulated body fluids(SBF).The inhibitory rates of PDA-coated Cu-MBG@PDA against Staphylococcus aureus and Escherichia coli both increase to over 99.90%,with excellent antibacterial properties.PDA-coated Cu-MBG@PDA has superior hydrophily,in vitro bioactive and antibacterial properties,and is expected to be used in the treatment of bone tissue defects and repair caused by chronic osteomyelitis.