硅酸盐根管封闭剂的体外细胞毒性、成骨潜力和抗菌活性评价

In vitro study on cytotoxicity,osteogenic potential and antibacterial activity of silicate root canal sealers

目的:评价新型硅酸锶基根管封闭剂C-Root SP的体外生物相容性和抗菌活性,为临床选择合适的根管封闭剂提供参考。方法:制备C-Root SP、i Root SP和AH Plus浸提液,体外培养L929细胞和MC3T3-E1细胞,比较3种封闭剂的细胞毒性和成骨潜力。将新鲜根管封闭剂与粪肠球菌溶液混合,利用直接接触实验(direct contact text,DCT)测定封闭剂的抗菌活性。采用SPSS 25.0软件包对数据进行统计学分析。结果:24、48、72 h时,各组封闭剂细胞毒性均有显著差异(P<0.01);与AH Plus相比,C-Root SP的细胞毒性更低(P<0.01)。C-Root SP促进细胞碱性磷酸酶活性方面优于AH Plus(P<0.01);促矿化结节形成能力方面i Root SP最强,C-Root SP次之,AH Plus最弱(P<0.01)。而C-Root SP可抑制粪肠球菌生长,其抑菌率显著高于AH Plus(P<0.01),C-Root SP与i Root SP对粪肠球菌的抑菌作用无显著差异(P>0.05)。结论:硅酸锶根管封闭剂C-Root SP具有较低的细胞毒性、一定的成骨潜力和对粪肠球菌的抗菌活性,可用于根管充填。

PURPOSE:To evaluate the in vitro biocompatibility and antibacterial activity of a new type of strontium silicate-based C-Root SP root canal sealer,and to provide a reference for clinical selection of sealers.METHODS:C-Root SP,iRoot SP and AH Plus extracts were prepared,L929 cells and MC3T3-E1 cells were cultured in vitro,and the cytotoxicity and osteogenic potential of the three sealers were compared.Fresh sealers were mixed with Enterococcus faecalis solution and the antibacterial activity of the sealer was determined by direct contact text(DCT).SPSS 25.0 software package was used for statistical analysis.RESULTS:At 24,48,and 72 h,the cytotoxicity of the sealers in each group were significantly different(P<0.01).Compared with AH Plus,the cytotoxicity of C-Root SP was lower(P<0.01).C-Root SP was superior to AH Plus in promoting the activity of alkaline phosphatase(ALP)(P<0.01).iRoot SP was the strongest in promoting the formation of mineralized nodules,followed by C-Root SP,and the weakest was AH Plus(P<0.01).C-Root SP inhibited the growth of Enterococcus faecalis,and its antibacterial rate was significantly higher than AH Plus(P<0.01).CONCLUSIONS:The strontium silicate root canal sealer C-Root SP has low cytotoxicity,certain osteogenic potential and antibacterial activity against Enterococcus faecalis,so it can be used for root canal filling.