miR-27a低表达对子宫内膜癌HEC-1-A细胞增殖的影响

The effect of low expression of miR-27a on the proliferation on endometrial cancer HEC-1-A cells

目的 研究miR-27a低表达对子宫内膜癌HEC-1-A细胞增殖的影响。方法 构建miR-27a低表达HEC-1-A细胞株。细胞实验分为空白对照组(HEC-1-A不做任何处理)、NC-miR组(转染miR-27a空载体)及miR-27a inhibitor组(转染miR-27a抑制剂)。qRT-PCR法检测各组细胞miR-27a表达情况;MTT比色法和平板克隆技术检测各组细胞增殖能力。结果 成功构建miR-27a低表达HEC-1-A细胞株。miR-27a inhibitor组miR-27a水平及细胞增殖能力均低于NC-miR组及空白对照组(P<0.05)。结论 miR-27a低表达抑制子宫内膜癌HEC-1-A细胞增殖能力。

Aim To study the effect of low miR-27a expression on the proliferation ability of HEC-1-A cells in human endometrial cancer.Methods Low expression of miR-27a HEC-1-A cell line was established.The experiment was set as blank control group(HEC-1-A without any treatment),NC-miR group(transfected empty vector)and miR-27a inhibitor group(transfected miR-27a inhibitor).The expression of miR-27a in each group of cells was detected by qRT-PCR.The cell proliferation capacity was detected by MTT colorimetric method and plate clone formation method.Results miR-27a low-expression HEC-1-A cell line was successfully constructed.The expression of miR-27a and the cell proliferation capacity in the miR-27a inhibitor group were significantly lower than those in NC-miR group and blank control group(P<0.05).Conclusion The low expression of miR-27a inhibits the proliferation of endometrial cancer HEC-1-A cells.