重组聚合酶等温扩增-侧流层析试纸条检测技术在诊断病原微生物中的应用研究进展

Advance in the application of recombinase polymerase amplification-lateral flow strips technology in the diagnosis of pathogenic microorganisms

重组聚合酶等温扩增(RPA)技术是新开发的具有高灵敏度、高特异性的等温扩增技术,将RPA与侧流层析试纸条检测(LFS)技术相结合,可快速鉴别目的基因。迄今为止,RPA-LFS技术已广泛运用于医药、食品、植物学等领域。本文将对RPA-LFS技术在病原微生物中的诊断进行综述,为实现病原微生物的即时诊断提供参考。

Recombinase polymerase amplification(RPA)is a newly developed isothermal amplification technology with high sensitivity and specificity.The combination of RPA and lateral flow strips(LFS)enables rapid identification of target genes.This technique has been widely used in medicine,food,botany,and other fields.This review generalizes the use of RPA-LFS technology for the diagnosing pathogenic microorganisms,providing a reference for point-of-care diagnosis of pathogenic microorganisms.