新型冠状病毒亚基因组RNA的产生及其检测方法

The generation and detection of 2019-nCoV subgenomic RNA

新型冠状病毒引起的新型冠状病毒感染(COVID-19)在全球范围广泛流行,对人类健康和世界经济造成了严重影响。新型冠状病毒是单股正链RNA病毒,可由复制转录复合物的不连续转录产生亚基因组RNA。这种不连续转录由转录调控序列调控,通过切换基因组RNA上的模板产生。目前检测亚基因组RNA的方法主要有二代测序技术、纳米孔测序技术、逆转录液滴数字聚合酶链反应和逆转录实时定量聚合酶链反应技术等。病毒进入感染细胞进行复制时才会产生亚基因组RNA,因此亚基因组RNA是较好的反映病毒复制的标志物。

The 2019 novel coronavirus(2019-nCoV)is a newly discovered pathogen in 2019.The coronavirus disease(COVID-19)has spread around the world and has greatly affected global health and the world economy.It is a positive-sense single-stranded RNA virus,which generates subgenomic RNA from discontinuous transcription of the replication-transcription complex(RTC).This discontinuous transcription is regulated by transcriptional regulatory sequences/elements,produced by switching templates on genomic RNA.At present,the detection methods of subgenomic RNA include the next generation sequencing,nanopore sequencing,reverse transcription dropletdigital polymerase chain reaction,reverse transcription real-time quantitative polymerase chain reaction,etc.Subgenomic RNA is produced only when the virus infects cell,so it may be a novel marker for viral replication.