半夏及其炮制品7种主要核苷类成分差异性分析

Differential analysis of 7 main nucleoside components in Pinellia ternata and its processed products

目的:核苷类成分是半夏的主要药效物质,通过比较半夏及其炮制品中主要核苷类成分含量的差异,为科学评价及有效控制半夏及其炮制品质量提供参考。方法:采用HPLC结合多变量统计分析,对半夏及其炮制品的主要核苷类成分进行含量测定,并筛选出炮制前后的差异性成分。结果:测定结果表明,生半夏中各核苷类化合物含量显著高于炮制品;法半夏中鸟苷、腺苷平均含量显著高于姜半夏(P<0.05),与清半夏各成分平均含量差异不显著;姜半夏和清半夏未见明显区分。主成分分析(PCA)结果显示生半夏及其炮制品可被明显区分;正交偏最小二乘判别分析(OPLS-DA)筛选得到生半夏与法半夏的差异性成分为尿苷、胸苷;生半夏与姜半夏的差异性成分为尿苷、鸟苷、腺苷、胸苷;生半夏与清半夏的差异性成分为尿嘧啶、尿苷、鸟苷、腺苷、胸苷;法半夏与姜半夏、法半夏与清半夏的差异性成分均为尿嘧啶、鸟苷、腺苷;姜半夏与清半夏未见明显区分。以上结果表明核苷类成分含量可体现半夏及其炮制品之间的差异。结论:该实验建立的含量测定方法科学、准确、稳定且易于操作,为半夏及其炮制品的质量控制提供了参考依据。

OBJECTIVE Nucleoside compounds are the main effective components of Pinellia ternata.To compare the differences in the contents of nucleoside components in Pinellia ternata and its processed products,so as to provide reference for scientific evaluation and effective quality control.METHODS HPLC combined with multivariate statistical analysis was used to determine the contents of nucleoside components in Pinellia ternata and its processed products,and the differential components of Pinellia ternata before and after processing were screened.RESULTS The results showed that the content of each component in the raw samples was significantly higher than that in the processed products.The average contents of guanosine and adenosine were higher in Pinelliae Rhizoma Praeparatum than those of Pinelliae Rhizoma Praeparatum Cum Zingibere Et Alumine(P<0.05).The mean content of each component was not significantly different from that of Pinelliae Rhizoma Praeparatum cum Alumine.There was no obvious difference between Pinelliae Rhizoma Praeparatum Cum Zingibere Et Alumine and Pinelliae Rhizoma Praeparatum cum Alumine.The results of the principal component analysis(PCA)showed that Pinellia ternata and its processed products could be significantly distinguished.The results of orthogonal partial least squares-discriminant analysis(OPLS-DA)showed uridine and thymidine were the differential components between Pinellia ternata and Pinelliae Rhizoma Praeparatum.Uridine,guanosine,adenosine and thymidine were the differential components between Pinellia ternata and Pinelliae Rhizoma Praeparatum Cum Zingibere Et Alumine.Uracil,uridine,guanosine,adenosine and thymidine were the differential components between Pinellia ternata and Pinelliae Rhizoma Praeparatum cum Alumine.The differences among Pinelliae Rhizoma Praeparatum,Pinelliae Rhizoma Praeparatum Cum Zingibere Et Alumine and Pinelliae Rhizoma Praeparatum Cum Alumine were uracil,guanosine and adenosine.No clear distinction was observed between Pinelliae Rhizoma Praeparatum cum Zingibere Et Alumine and Pinelliae Rhizoma Praeparatum Cum Alumine.The results showed that the contents of nucleosides reflected the differences between Pinellia ternata and its processed products.CONCLUSION The content determination method established is scientific,accurate,stable and convenient to operate,which provides reference for the quality control of Pinellia ternata and its processed products.