羊角天麻抑菌作用及其活性部位初步研究

Preliminary Study on Antibacterial Effect and Active Parts of Dobinea delavayi

目的:探讨羊角天麻的抑菌作用及其活性部位。方法:提取羊角天麻水部位、石油醚部位、乙酸乙酯部位、正丁醇部位、30%醇沉部位、60%醇沉部位、95%醇沉部位,采用琼脂平板二倍稀释法测定以上7个部位对耐甲氧西林金黄色葡萄球菌(MRSA)、金黄色葡萄球菌、粪肠球菌的最低抑菌浓度,并探讨温度、紫外线对羊角天麻不同活性部位抑菌活性的影响。结果:羊角天麻水部位对MRSA、金黄色葡萄球菌、粪肠球菌有抑制作用,60%醇沉部位、95%醇沉部位对MRSA、金黄色葡萄球菌有抑制作用,抑菌圈直径分别为(21.2±0.6)、(20.4±0.1)、(19.6±0.6)、(20.6±0.1)、(22.2±0.2)、(17.5±0.2)、(20.3±0.3)mm,石油醚部位、乙酸乙酯部位、正丁醇部位无抑菌活性。此外,羊角天麻水部位、60%醇沉部位、95%醇沉部位对高温和紫外线敏感。结论:羊角天麻具有确切的体外抑菌活性,水部位是抑制MRSA、粪肠球菌的主要活性部位,60%乙醇沉部位是抑制金黄色葡萄球菌的主要活性部位,该研究为羊角天麻的综合利用与开发提供了理论依据。

Objective:To investigate the antibacterial effect and active parts of Dobinea delavayi.Methods:The water,petroleum ether,ethyl acetate,n-butanol,30%ethanol precipitation,60%ethanol precipitation,and 95%ethanol precipitation parts of D.delavayi were extracted,and the minimum inhibitory concentration of the above 7 parts against methicillin resistant Staphylococcus aureus(MRSA),Staphylococcus aureus and Enterococcus faecalis were measured by agar plate double dilution method.The effects of temperature and ultraviolet on the antibacterial activity of different active parts of D.delavayi were also investigated.Results:The water part of D.delavayi had an inhibitory effect on MRSA,S.aureus,and E.faecalis.The 60%ethanol-precipitated part and 95%ethanol-precipitated part had an inhibitory effect on MRSA and S.aureus,with antibacterial zone diameters of(21.2±0.6),(20.4±0.1),(19.6±0.6),(20.6±0.1),(22.2±0.2),(17.5±0.2),(20.3±0.3)mm,respectively.The petroleum ether,ethyl acetate,and n-butanol parts had no antibacterial activity.In addition,the water part,the 60%ethanol-precipitated part,and the 90%ethanol-precipitated part of D.delavayi were sensitive to high temperature and ultraviolet.Conclusion:D.delavayi has definite antibacterial activity in vitro.The water part is the main active part for inhibiting MRSA and E.faecalis,and the 60%ethanol-precipitated part is the primary active part for inhibiting S.aureus.This study provides a theoretical basis for the comprehensive utilization and development of D.delavayi.