流式细胞分选仪研究进展与展望

Advances and prospect of flow cytometer

流式细胞技术一直是生物细胞研究的重要手段,近年来流式细胞分选仪在细胞富集、筛选后再利用等方面的应用使得对能够满足高通量、高细胞信息提取、高细胞活性要求的流式细胞分选仪的需求增加。本文阐述基于光学探测方法的流式细胞分选仪结构、工作原理及其发展现状出发;提出在光学流式细胞分选仪研究领域出现无标记化和微流控芯片化两种新的发展趋势。在两种发展趋势下,无标记成像方法、微流控片上分选系统和片上压电致动器集成分选等新兴技术越来越多地应用到光学流式细胞分选仪的研发设计中,本文从技术特性、性能优势、应用前景等方面对这些技术的研究进展以及面临的挑战进行了总结和展望,供流式细胞分选技术的使用者及研究者参考。

Flow cytometry has always been an essential means for biological cell research.In recent years,the applications of flow cytometer for cell enrichment and post-screening reuse have led to an increased demand for flow cytometers capable of high throughput,high cell information extraction as well as high cell activity.Herein the structure,working principle and current development status of flow cytometer based on optical detection methods are overviewed.In order to meet the new application demands and overcome the inherent defects of the current commercial optical flow cytometer,the trends of labelfree and microfluidics chip emerge in optical flow cytometer.The emerging technologies such as label-free imaging methods,microfluidics on-chip sorting systems,and on-chip piezoelectric actuator integrated sorting are increasingly applied to the research and design of optical flow cytometer.The research advances and challenges of these technologies are summarized from the aspects of technical characteristics,performance advantages,application prospects,etc.,for providing users and researchers with reference.