病原微生物高通量测序检测确诊耻垢分枝杆菌感染1例

A case of Mycobacterium smegmatis infection confirmed by next-generation sequencing technology

耻垢分枝杆菌属于Ⅳ型(快速生长型)非结核分枝杆菌,广泛分布于自然环境中,可以在皮肤黏膜正常存在,当机体免疫力下降或正常皮肤黏膜破损时可成为条件致病菌。耻垢分枝杆菌感染相关病例报道甚少,发病率低,临床上容易出现漏诊。应用病原微生物高通量测序技术(NGS)对感染性疾病的病原体筛查能更早期发现病原体,达到早诊断、早治疗的目的,极大提高了疑难性感染性疾病的诊断率。本文通过血液NGS检测确诊1例耻垢分枝杆菌感染病例进行报道并文献复习,拟提高对耻垢分枝杆菌感染的诊治水平。

Mycobacterium smegmatis belongs to type IV(rapid growth) non-tuberculous mycobacteria.It is widely distributed in the natural environment and can exist normally in the skin and mucous membranes.When the immunity of the body is weakened or the normal skin and mucous membranes are damaged.It can become an opportunistic pathogen.There are few cases of Mycobacterium smegmatis infection reported,the incidence is low,and it is easy to be missed clinically.Pathogenic microorganism high-throughput sequencing technology(next-generation sequencing technology,NGS) can extract hundreds of thousands to millions of DNA and RNA molecules at a time.The application of this technology enables clinicians to identify pathogens of infectious diseases.Screening can detect pathogens earlier,achieve the purpose of early diagnosis and early treatment,and greatly improve the diagnosis rate of difficult infectious diseases.In this paper,a case of Mycobacterium smegmatis infection was diagnosed by blood NGS detection to report and review the literature,in order to improve the ability of diagnosis and treatment of Mycobacterium smegmatis infection.