DDAH1基因836A/T多态性与云南地区汉族2型糖尿病肾脏疾病的相关性分析

Correlation between DDAH1 Gene 836A/T Polymorphism and Type 2 Diabetic Kidney Disease in Yunnan Han Population

目的探讨DDAH1基因836A/T多态性与云南地区汉族人群2型糖尿病肾脏疾病发生的相关性。方法选取T2DM患者共660例,根据UACR检测结果,将患者分为3组:单纯2型糖尿病组(DN0组),合并早期肾病组(DN1组),合并临床期肾病组(DN2组)。健康人群(NC组)304例。采用PCR-DNA测序技术对DDAH1基因836A/T多态性进行基因分型,ELISA法检测血浆非对称性二甲基精氨酸(ADMA)浓度。结果DDAH1基因836位点AA基因型在DN1+DN2组的分布频率高于DN0组(P<0.05)。A等位基因在DN1+DN2组的分布频率高于DN0组(P<0.05)。T2DM患者中AA基因型携带者较AT+TT基因型个体具有更高的ADMA水平。ADMA水平在DN1+DN2组高于DN0组(P<0.05)。通过相关危险因素分析发现,在T2DM患者中ADMA水平、DDAH1基因836位点AA基因型是发生DKD的危险因素。结论DDAH1基因836位点AA基因型可能是云南地区汉族T2DM患者发生DKD的危险因素,AA基因型携带者ADMA水平升高。

objective To investigate the association between DDAH1 gene 836A/T polymorphism and type 2 diabetic kidney disease(DKD)in Yunnan Han population.Methods A total of 660 patients with T2DM were included.Patients were divided into three groups based on UACR results:simple type 2 diabetes mellitus group(DN0 group),complicated with early nephropathy group(DN1 group),and complicated with clinical nephropathy group(DN2 group).A total of 304 healthy people were included as normal control group(NC group).The genotype of DDAH1 gene 836A/T polymorphism was determined by PCR-DNA sequencing.The plasma asymmetric dimethylarginine(ADMA)concentration was measured by ELISA.Results The distribution frequency of AA genotype and allele A at the 836 locus of DDAH1 gene in the DN1+DN2 group was significantly higher than those in the DN0 group(P<0.05).AA genotype carriers have higher ADMA levels than AT+TT genotype individuals in T2DM patients.The level of ADMA in DN1+DN2 group was higher than that in DN0 group(P<0.05).Logistic regression analysis showed that ADMA level and DDAH1 gene 836 AA genotype were risk factors for DKD in T2DM patients.Conclusions The DDAH1 gene 836 AA genotype may be a risk factor for diabetic kidney disease in T2DM patients of Han nationality in Yunnan Province,and patients with this genotype have higher ADMA levels.