手掌接触性生物检材DNA检出率与汗孔密度的相关性研究

Correlation between DNA Detection Rate and Sweat Pore Density of Palm Touch Biological Samples

目的研究男性手掌接触性生物检材的DNA(即接触性DNA)检出率与汗孔密度的关系,探索影响接触性DNA检出率的因素。方法使用加层捺印法采集73名男性大学生右手小鱼际掌印,对限定区域进行汗孔计数;采用手掌直接擦拭法和载玻片转印间接擦拭法转移35名受试者的手掌接触性生物检材,使用直接扩增法对检材进行PCR,并对STR结果进行分析;对2种转移方法的STR等位基因检出率与汗孔密度进行相关性分析。结果综合汗孔采集成功率为84.93%,汗孔密度为(531.41±97.69)个/cm^(2),最小值为309个/cm^(2),最大值为814个/cm^(2);35名受试对象的手掌汗孔密度和直接擦拭法、间接擦拭法的STR等位基因检出率之间均不存在相关关系(P1=0.806,P2=0.701)。结论通过2种不同的实验方法对手掌接触性生物检材进行DNA检验,均表明手掌汗孔密度与接触性DNA的检出率无相关性,说明汗孔密度不是接触性DNA检出率的主要影响因素,或不是其唯一影响因素。

Objective To study the relationship between the detection rate of touch DNA and sweat pore density in male palm touch biological samples,and explore the factors affecting the detection rate of touch DNA.Methods The right hand hypothenar palmprints of 73 male college students were collected by using the added layer fingerprinting method,and the sweat pore count was performed in specific site.Palm-touch biological samples of 35 individuals were collected by direct palm wiping method and indirect glass slide transfer wiping method.PCR was performed on the samples by direct amplification,and short tandem repeat(STR)results were analyzed.The correlation between STR allele detection rate and sweat pore density was analyzed.Results The success rate of comprehensive sweat pore collection was 84.93%,the density of the sweat pore was(531.41±97.69)/cm^(2),the minimum value was 309/cm²,and the maximum value was 814/cm^(2).There was no correlation between the palm sweat pore density and the STR allele detection rates using direct and indirect wiping methods(P1=0.806,P2=0.701).Conclusion Two different experimental methods were used to test the DNA of the palm touch biological samples,both of which showed that there was no correlation between the sweat pore density and the detection rate of touch DNA,indicating that the sweat pore density was not the main or only influencing factor of the detection rate oftouch DNA.