含ACC脱氨酶的根际促生菌对切花月季生长的影响

Effect of Rhizobacteria Containing ACC Deaminase on Growth of Rose Bush

【目的】探明含ACC脱氨酶(1-aminocyclopropane-1-carboxylate deaminase)的不同植物根际促生菌对切花月季白荔枝的促生效果。【方法】以白荔枝为试验材料,在其盆栽过程中施入含ACC脱氨酶的不同PGPR菌株,通过测定其株高、茎粗、分枝数等农艺性状、叶绿素a/b值、叶片保护酶活性及MDA含量、花朵乙烯释放量、光合及叶绿素荧光参数等生理指标,探讨不同菌株对白荔枝生长及生理的影响。【结果】施入含ACC脱氨酶的不同PGPR菌株对白荔枝生长及光合作用产生的影响有所差异,其中菌株F23和F195处理较对照株高增加25.9%和26.0%,且F23处理后茎粗比对照处理增加17.1%,菌株F23和F195处理叶绿素a/b也高于对照。菌株F23显著提高了根际土壤脲酶、磷酸酶和蔗糖酶活性至42.6%、 16.3%和48.8%;且菌株F23对POD以及CAT活性的提高有显著促进作用,分别达到对照的1.78和2.07倍,花朵乙烯释放量比对照减少37.8%。净光合速率经菌株F195和F23处理后显著高于对照,分别达对照的1.40倍和1.16倍。【结论】综合比较认为,菌株F23对切花月季白荔枝的促生效果最好。

【 Objective】 Effect of plant growth promoting rhizobacteria(PGPR) containing ACC deaminase on the productivity of rose bushes was studied.【Method】 A pot experiment was conducted on White Lichee,a variety of roses commonly used commercially for cut flowers.Rhizobacteria carrying the plant growth promoter,ACC deaminase,were added to the soil,and effects on the agronomic traits,such as plant height,stem girth,and number of branches,as well as the physiological indexes,such as chlorophyll a/b,leaf protective enzyme,MDA content,flower ethylene release,photosynthesis,and chlorophyll fluorescence,of the plants monitored.【Result】 In comparison with control,the incorporation of F23 or F195PGPR,respectively,resulted in the rose bushes 25.9% and 26.0% taller with higher contents of chlorophyll a and chlorophyll b;F23,17.1% larger stem girth,significantly greater activities of urease(by 42.6%),phosphatase(by 16.3%),and sucrase(by48.8%) in rhizosphere soil,and POD(1.78x of control) and CAT(2.07x of control) in leaf.The floral ethylene release of the bushes treated with F23 was 37.0% lower than control,while the net photosynthetic rate of the F195-treated bushes significantly rose to 1.40 times and that of F23-treated bushes 1.16 times of control.【Conclusion】 Overall,F23 appeared to most significantly promote the growth of White Lichee rose bushes among different PGPR tested.