蚕豆芽苗菜高左旋多巴产量的LED光培养条件研究

LED-stimulated Levodopa Production in Broad Bean Sprouts

【目的】研究不同LED光培养条件对蚕豆芽苗菜功效成分左旋多巴产量的影响。【方法】通过单因素试验和L9(34)正交试验研究不同光强、光照时间和培养时间3个因素对蚕豆芽苗菜生长及其功效物质左旋多巴产量的影响。【结果】弱光促进蚕豆芽苗菜干物质的积累,强光促进蚕豆芽苗菜左旋多巴质量分数的积累,蚕豆芽苗菜高左旋多巴产量的最优培养条件为光强500 lx、光照时间9 h、培养时间6 d,在此培养条件下蚕豆芽苗菜左旋多巴产量为19.62 g·m^(-2)。【结论】通过调节蚕豆芽苗菜的光培养条件可以提高其功效物质左旋多巴的产量,本研究为蚕豆芽苗菜的功效物质成分提取提供参考依据,有利于蚕豆芽苗菜的开发利用。

【Objective】Effect of LED photoculture on yield of levodopa in broad bean sprouts was studied.【Method】Broad bean sprouts were exposed to LED of varied intensity and cultured for varied time periods in single factor and L9(34)orthogonal experiments.Levodopa generated in the sprouts under the conditions was determined for process optimization.【Result】Low LED light enhanced the accumulation of sprout dry matters.However,it was under high intensity the productivity of levodopa in the sprouts was significantly encouraged.The optimum conditions for a maximized levodopa production of 19.62 g·m^(-2) were found to include 9 h of 500 lx LED exposure in 6 d of cultivation.【Conclusion】The photoculture of broad bean sprouts under intense LED lights could significantly promote the production of the functional amino acid,levodopa.