Box-Behnken响应面优化超声辅助提取藿香蓟多酚及抗氧化活性研究

Optimization of ultrasonic-assisted extraction of polyphenols from Ageratum conyzoides L.by Box-Behnken response surface and evaluation of antioxidation activity

试验旨在研究藿香蓟多酚的最佳提取工艺及其抗氧化活性。在单因素试验基础上,采用Box-Behnken响应面法优化藿香蓟多酚的最佳提取工艺,采用体外法测定藿香蓟多酚的抗氧化能力。结果显示,超声辅助提取藿香蓟多酚的最优工艺为乙醇浓度35%、提取时间30 min、液料比40 mL/g、提取温度50℃,在此条件下藿香蓟多酚得率为5.32%。当藿香蓟多酚浓度为4.5 g/L时,对2, 2’-联氨-双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)、1, 1-二苯基-2-三硝基苯肼(DPPH)自由基清除率分别为93.4%、96.2%,还原能力接近浓度为0.8 g/L的维生素C (VC)。研究表明,藿香蓟多酚具有较强的抗氧化能力,为开发藿香蓟新型天然抗氧化剂在动物生产中的应用提供参考。

The experiment was to study optimal extraction process and antioxidant activity of polyphenols from Ageratum conyzoides L.on basis of single factor experiment,Box-Behnken response surface methodology was used to optimize optimal extraction process of Ageratum conyzoides L.polyphenols.The antioxidant capacity of Ageratum conyzoides L.polyphenols was measured in vitro.The results showed that the optimal process for ultrasound-assisted extraction of polyphenols from Ageratum conyzoides L.was ethanol concentration of 35%,extraction time of 30 min,liquid-solid ratio of 40 mL/g and extraction temperature of 50℃.Under these conditions,the yield of polyphenols from Ageratum conyzoides L.was 5.32%.At a concentration of 4.5 g/L,the scavenging rates of ABTS and DPPH free radical were 93.4%and 96.2%,respectively,and reducing ability was close to that of VC at a concentration of 0.8 g/L.The study indicates that the Ageratum conyzoides L.polyphenols has strong antioxidant capacity,which provide a reference for the development of new natural antioxidants of Ageratum conyzoides L.in animal production.