摘要
本研究探讨线粒体靶向抗氧化剂Mito-Tempo对过氧化氢诱导猪卵母细胞线粒体损伤修复的影响。在猪卵母细胞体外成熟过程中添加10 mmol·L^(-1)过氧化氢或过氧化氢+0.1μmol·L^(-1)Mito-Tempo,检测卵丘细胞扩散、卵母细胞成熟及线粒体功能相关因子,观察孤雌胚胎发育情况。结果表明,与过氧化氢组相比,添加Mito-Tempo可显著提高卵丘细胞扩散指数,显著降低Mito SOX水平,提高膜电位及线粒体功能基因表达水平,显著改善猪卵母细胞体外成熟率及孤雌胚胎发育能力(P<0.05)。证明Mito-Tempo对过氧化氢诱导的猪卵母细胞线粒体损伤具有修复功能。
To investigate the effect of Mito-Tempo on H2O2-induced mitochondrial dysfunction in porcine oocytes,supplemented 10 mmol·L^(-1) H_(2)O_(2) and/or 0.1μmol·L^(-1) Mito-Tempo to oocyte maturation medium,evaluated the cumulus cell expansion,oocyte maturation and mitochondria-relative factors,and obsened subsequent embryo development competence after parthenogenetic activation.The results showed that Mito-Tempo significantly improved cumulus expansion index compared to the H_(2)O_(2) group(P<0.05),Mito-Tempo addition significantly reduced the level of MitoSOX,and increased the mitochondrial membrane potential and mitochondria-relative gene expression(P<0.05),additionally,Mito-Tempo significantly improved porcine oocyte maturation rate in vitro and embryo developmental competence(P<0.05).In conclusion,Mito-Tempo could rescue the mitochondrial dysfunction from H_(2)O_(2)-induced damage during porcine in vitro oocyte maturation.
作者
金君学
綦新月
刘子瑜
张吉龙
马祥钧
孙婧陶
JIN Junxue;QI Xinyue;LIU Ziyu;ZHANG Jilong;MA Xiangjun;SUN Jingtao(Key Laboratory of Animal and Cell Genetic Engineering of Heilongjiang Province,School of Life Science,Northeast Agricultural University,Harbin 150030,China)
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2023年第6期28-34,共7页
Journal of Northeast Agricultural University
基金
国家重点研发计划项目(2021YFA0805902)
国家自然科学基金青年基金项目(32002179)
黑龙江省自然科学基金优秀青年项目(YQ2020C007)
国家级大学生创新创业训练计划(202210224131)。