摘要
以解脂耶氏酵母(Yarrowia lipolytica)、巴斯德毕赤酵母(Pichia pastoris)、马克斯克鲁维酵母(Kluyveromyces marxianus)、圆红冬孢酵母(Rhodosporidium toruloides)、多形汉逊酵母(Hansenula polymorpha)为代表的非常规酵母凭借较广的底物利用谱、较强的环境耐受性等优势,已成功实现多种天然产物的高效生产。随着合成生物学及基因编辑技术的发展,针对非常规酵母代谢工程改造的工具和策略也逐渐丰富。本文介绍了几类常见的非常规酵母的生理特性、工具开发及应用现状,并总结归纳了天然产物合成优化中常用的代谢工程策略;最后讨论了现阶段非常规酵母作为天然产物合成细胞工厂的优势和不足,并对后续研究和发展趋势进行了展望。
Non-conventional yeasts such as Yarrowia lipolytica,Pichia pastoris,Kluyveromyces marxianus,Rhodosporidium toruloides and Hansenula polymorpha have proven to be efficient cell factories in producing a variety of natural products due to their wide substrate utilization spectrum,strong tolerance to environmental stresses and other merits.With the development of synthetic biology and gene editing technology,metabolic engineering tools and strategies for non-conventional yeasts are expanding.This review introduces the physiological characteristics,tool development and current application of several representative non-conventional yeasts,and summarizes the metabolic engineering strategies commonly used in the improvement of natural products biosynthesis.We also discuss the strengths and weaknesses of non-conventional yeasts as natural products cell factories at current stage,and prospects future research and development trends.
作者
钱芷兰
宋丽丽
刘启
龚秀龙
康艺嘉
何子雨
龙浩雨
蔡孟浩
QIAN Zhilan;SONG Lili;LIU Qi;GONG Xiulong;KANG Yijia;HE Ziyu;LONG Haoyu;CAI Menghao(School of Biotechnology,East China University of Science and Technology,Shanghai 200237,China;State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China)
出处
《生物工程学报》
CAS
CSCD
北大核心
2023年第6期2284-2312,共29页
Chinese Journal of Biotechnology
基金
国家重点研发计划(2022YFC2805102,2020YFA0907804)
国家自然科学基金(31870073,32201206)。
关键词
天然产物
非常规酵母
细胞工厂
代谢工程
natural products
non-conventional yeasts
cell factories
metabolic engineering
