一株2,4-二氯苯酚降解菌的筛选及降解特性

Screening of a 2,4-dichlorophenol-degrading bacteria and its biodegradation characteristics

从活性污泥中分离筛选出一株2,4-二氯苯酚(2,4-DCP)降解菌,结合16S rDNA对菌株进行鉴定,并考察了菌株对2,4-DCP的耐受性能及降解特征.结果表明,该降解菌为棒状杆菌属(Corynebacterium sp.),命名为ZH404;该菌对2,4-DCP的最小抑菌浓度可达160 mg/L;在无机盐培养基中菌株ZH404培养30 h生物量达到0.29,添加5%和10%葡萄糖作为辅助碳源,生物量分别提高了1.3和1.9倍;该菌处理2,4-DCP(50 mg/L)10 d降解率达到40.1%,添加5%和10%葡萄糖的降解率分别提高到46.4%和63.5%.可见菌株ZH404能够在以2,4-DCP为唯一碳源的环境下生长,且辅助碳源有利于ZH404的生长及提高对2,4-DCP的降解效率,该菌株可用于含酚类污染物的有机废水处理.以上结果可为实际含酚废水的生物修复提供理论依据和技术参考.

A strain of 2,4-dichlorophenol(2,4-DCP)degrading bacteria was isolated and screened from activated sludge,and then was identified by 16S rDNA sequence analysis.The tolerance and degradation characteristics were investigated.The results showed that a DCP-degrading bacterial strain ZH404 was isolated and identified as Corynebacterium sp.Its minimum inhibitory concentration to 2,4-DCP was 160 mg/L.The biomass of strain ZH404 in inorganic salt medium reached 0.29 at the 30th hour,and increased by 1.3 and 1.9 times with addition of 5% and 10% glucose as auxiliary carbon source.Treating with strain ZH404 for 10 days,the degradation rate of 2,4-DCP(50 mg/L)reached 40.1%,and increased to 46.4%and 63.5%,with 5% and 10% glucose,respectively.It indicated that the strain ZH404 could grow with 2,4-DCP as the sole carbon source,and also grow better with the addition of the auxiliary carbon source,resulting in higher degradation efficiency.It presented the strain ZH404 could be used for the treatment of chlorophenol wastewater.The above results provided theoretical basis and technical reference for the bioremediation of phenols wastewater.