马铃薯“费乌瑞它”愈伤组织诱导及分化技术

Callus Induction and Differentiation Technology of Potato "Favorita"

为建立马铃薯植株的离体再生体系,以“费乌瑞它”为材料,开展了不同激素配比对试管苗的继代、愈伤组织诱导及分化的影响研究。结果表明,试管苗继代培养适宜的激素配比为6-BA 0.20 mg·L^(-1)+NAA 0.50 mg·L^(-1)+ZT 0.50 mg·L^(-1),生根率75%,平均苗高2.757 cm;茎段愈伤组织最佳诱导激素配比为NAA 1.50 mg·L^(-1)+2, 4-D 1.00 mg·L^(-1)+6-BA 0.50 mg·L^(-1),诱导率达到92.00%;使用相同的激素组合诱导茎段和叶片愈伤组织,诱导率分别为89.66%和71.44%;愈伤组织分化最优激素配比为6-BA 1.00 mg·L^(-1)+GA31.00 mg·L^(-1)+ZT 1.00 mg·L^(-1),分化率为33.33%。初步建立的马铃薯“费乌瑞它”植株的离体再生体系可用于后续马铃薯遗传转化及基因编辑研究。

In order to establish the ex vivo regeneration system of potato plants,the effects of different hormone ratios on the succession,callus induction and differentiation of test tube seedlings were studied using"Favorita"as the material.The results showed that the suitable hormone ratio for IVF seedling subculture was 6-BA 0.20 mg·L^(-1)+NAA 0.50 mg·L^(-1)+ZT 0.50 mg·L^(-1),rooting rate was 75%,and the average seedling height was 2.757 cm.The optimal induction hormone ratio of st em callus was NAA 1.50 mg·L^(-1)+2,4-D 1.00 mg·L^(-1)+6-BA 0.50 mg·L^(-1),and the induction rate reached 92.00%.The same hormone combination was used to induce callus of stem segment and leaf,and the induction rates were 89.66%and 71.44%,respectively.The optimal hormone ratio for callus differentiation was 6-BA 1.00 mg·L^(-1)+GA 31.00 mg·L^(-1)+ZT 1.00 mg·L^(-1),and the differentiation rate was 33.33%.The preliminary established in vitro regeneration system of potato"Favorita"plants can be used for subsequent potato genetic transformation and gene editing research.