神经母细胞瘤中MYCN相关长链非编码核糖核酸的功能研究

Function Analysis of Long Noncoding RNA Associated with Expression of MYCN in Neuroblastoma

目的:筛选神经母细胞瘤(NB)中MYCN相关的长链非编码核糖核酸(lncRNA),分析其功能及对患者预后的影响,寻找潜在治疗靶点和分子标志物。方法:分别从TARGET及GEO数据库下载基因表达数据,并将两组数据分成MYCN高表达组和低表达组,R语言筛选出两组差异表达lncRNA,取共同差异表达lncRNA;通过ENCORI数据库预测与lncRNA互作的核糖核酸(RNA),DAVID数据库分析相关基因的功能与信号通路的富集情况;TARGET数据库进行相关生存分析。结果:TARGET数据库中共筛选出差异倍数在两倍以上的lncRNA 1104个,GEO数据库筛选出差异基因2849个,两个数据库中共同差异表达lncRNA 59个,ENCORI数据库预测与这些基因互作mRNA 547个,功能分析发现这些基因主要富集在调控神经系统疾病的相关通路,并通过调控转录、蛋白绑定等发挥相关生物学功能。利用TARGET数据库进行生存分析发现其中VPS9D1-AS1、LINC00649、LINC01234的异常表达可以影响患者生存。结论:运用生物信息学通过对MYCN相关差异表达lncRNA的分析发现VPS9D1-AS1、LINC00649、LINC01234可能与NB的发生发展密切相关,可为后续的研究提供方向与思路。

Objective To screen long noncoding RNA(lncRNA)associated with MYCN expression in neuroblastoma(NB),analyzing its effect on the patients'prognosis,and finding potential therapeutic targets.Methods Gene expression data were downloaded from TARGET and GEO databases,and the two groups were divided into MYCN high expression group and low expression group,respectively.R language was used to screen the differentially expressed genes,and obtained common differentially expressed lncRNAs.The mRNAs interacting with those lncRNAs were predicted by public ENCORI database;DAVID database was used to analyze the functions of these genes.Survival analysis of the differentially expressed lncRNAs was performed by TARGET database.Results A total of 1104 the differentially expressed lncRNAs were screened out from TARGET database,2849 differentially expressed genes were screened from geo database,59 common differentially expressed lncRNAs were identified after intersection with GEO identified;and 547 mRNAs were predicted to interact with them by ENCORI database.These differentially expressed lncRNAs were significantly enriched in the protein binding,negative regulation of transcription from RNA polymerase II promoter,and Alzheimer disease signaling pathway.TARGET database was used for further survival analysis,showed that VPS9D1-AS1,LINC00649 and LINC01234 could affect the survival of patients.Conclusion Through bioinformatics analysis of differentially expressed lncRNAs related to MYCN expression,it was found that VPS9D1-AS1,LINC00649 and LINC01234 may be closely related to the occurrence and development of NB,providing direction and ideas for subsequent research.