高通量测序技术在重症肺部感染中的应用价值

Application Value of High-throughput Sequencing Technology in Patients with Severe Pulmonary Infection

目的分析高通量测序技术在重症肺部感染患者中的应用效果。方法选取2021年4月至2022年4月本院收治的84例重症肺部感染患者,按随机数表法分为两组,各42例。对照组采用传统的病原体培养检测,观察组采用高通量测序技术检测。对比两组肺泡灌洗液与外周血标本内的病原体检出情况、抗生素总使用强度、入住重症监护病房(ICU)时间与28 d内病死率。结果观察组肺泡灌洗液内的病原体检出率为59.52%(25/42),高于对照组的23.81%(10/42);抗生素总使用强度为(135.26±5.69),低于对照组的(189.63±8.71);入住ICU时间为(12.13±1.71)d,短于对照组的(16.41±3.59)d,差异有统计学意义(P<0.05)。两组外周血标本内的病原体检出率、28 d内病死率相比,差异无统计学意义(P>0.05)。结论高通量测序技术在重症肺部感染患者检查中效果显著,能够提高病原体检出率,减少抗生素总使用强度与入住ICU时间,且28d内病死率较低,临床应用价值较高,可进行大力推行。

Objective To analyze the effect of high-throughput sequencing technology in patients with severe pulmonary infection.Methods 84patients with severe pulmonary infection admitted to our hospital from April 2021to April 2022were randomly divided into two groups,42patients in each group.The control group was detected by traditional pathogen culture,and the observation group was detected by high-throughput sequencing technology.The pathogen detection,total antibiotic use intensity,time in intensive care unit(ICU)and mortality within 28days in alveolar lavage fluid and peripheral blood samples were compared between the two groups.Results The detection rate of pathogens in the alveolar lavage fluid in the observation group was 59.52%(25/42),higher than 23.81%(10/42)in the control group,the total use intensity of antibiotics was(135.26±5.69),lower than(189.63±8.71)in the control group,and the stay time in the ICU was(12.13±1.71)days,shorter than(16.41±3.59)days in the control group(P<0.05);However,there was no significant difference between the two groups in the detection rate of pathogens in peripheral blood samples and the mortality rate within 28days(P>0.05).Conclusion High throughput sequencing technology has a significant effect in the examination of patients with severe pulmonary infection.It can improve the detection rate of pathogens,reduce the total use intensity of antibiotics and the time spent in ICU,and has a low mortality rate within 28days.It has a high clinical application value and can be vigorously promoted.