摘要
This article aims to explain the inhibitory mechanism of thinned-young apple polyphenols(YAP)toward Fusobacterium nucleatum(F.nucleatum),Porphyromonas gingivalis(P.gingivalis)and Prevotella intermedia(P.intermedia).YAP has optimal antibacterial concentration against F.nucleatum(10.00 mg/mL),P.gingivalis(8.00 mg/mL)and P.intermedia(8.00 mg/mL),with viability of 47.97%,38.01%and 36.94%,respectively.Scanning electron microscopy(SEM)and confocal laser scanning microscopy(CLSM)observation revealed that YAP could cause the morphological changes of the halitosis-related bacterial cells and induce cell apoptosis.With the increase of YAP treatment concentrations,the permeability of the outer membranes(OM)and inner membranes(IM)of halitosis-related bacteria dramatically increased,resulting in the release of proteins and nucleic acids.In addition,bacterial cell membrane potential(MP)decreased after exposure to YAP.The results of this study provide new antibacterial agents for halitosis-related bacteria and find a new way for the development of thinned-young apples.
基金
supported by the National Natural Science Foundation of China(No.31701563)
the Key Research and Development Program of Shaanxi Province(No.2019NY-124)
Ministry of Education(XGZX 2021G08)
the Fundamental Research Funds for the Central Universities at Shaanxi Normal University(1301031057).
