摘要
Difenoconazole is triazole fungicide,widely used to prevent the growth and reproduction of fungi and to control fungal diseases in fruits and vegetables.In this work,we prepared a hapten of difenoconazole and produced a highly-sensitive anti difenoconazole-monoclonal antibody(mAb)with an IC50 of 0.64 ng/mL.Using the mAb,an immunochromatographic assay(ICA)was developed to analyze difenoconazole residues in pear and cucumber samples.The ICA exhibited LOD values of 2.60 and 2.30 ng/g for difenoconazole in pear and cucumber samples,respectively.The ICA showed average recoveries of difenoconazole ranging from 94.3%±3.6%–105.4%±1.6%,and CVs of 1.5–4.2%in cucumber and pear samples.When used to measure difenoconazole in samples,the ICA results were compatible with those detected by LC/MSMS.The results of this study support the idea that an ICA is a practical and effective tool for high-throughput and rapid analysis of difenoconazole residues in vegetables and fruits.
基金
supported by the National Key Research and Development Program of China(2019YFC1604503).