基于GEO数据库对成人急性髓系白血病的生物信息学分析

Bioinformatic analysis of adult acute myeloid leukemia based on GEO database

目的 利用生物信息学挖掘成人急性髓系白血病(acute myeloid leukemia, AML)的关键基因并探索其发病机制。方法 从GEO数据库下载成人AML和健康对照的基因芯片,利用R语言筛选出两者差异表达基因,利用DAVID数据库对差异表达基因进行GO分析和KEGG通路富集分析。基于STRING数据库及Cytoscape软件构建蛋白质相互作用网络,分析其关键基因簇及关键基因。结果 共纳入3个平台的成人AML和健康对照的基因芯片,筛选出共同差异表达基因85个,包括16个上调基因和69个下调基因。富集分析显示共同差异表达基因主要集中在免疫反应、蛋白结合、质膜上,主要涉及细胞因子-细胞因子受体相互作用、造血细胞谱系、T细胞受体信号通路、自然杀伤细胞介导的细胞毒性和癌症中的转录失调等信号通路。蛋白质相互作用网络提示存在3个主要的基因簇和10个关键基因,分别为CD2、CCR7、PRF1、CD247、GZMB、GZMA、KLRB1、CD3D、IL2RB和CCL5。结论 筛选出的关键基因和信号通路有助于加深对成人AML发病机制的理解,同时为后续研究提供一定的理论依据。

Objective This paper aims to identify the key genes and explore the development mechanisms of adult acute mye⁃loid leukemia(AML)by bioinformatic analysis.Methods The gene chips of adult AML and healthy controls were downloaded from GEO database,and the differentially expressed genes were screened by R language.GO analysis and KEGG pathway enrichment analysis of differential expressed genes were performed using DAVID database.A protein-interaction network was constructed based on STRING database and Cytoscape software to analyze key gene clusters and key genes.Results A total of 3 platforms of adult AML and healthy controls gene chips were included,and 85 differentially expressed genes with intersection in this 3 data platforms were selected,including 16 up-regulated genes and 69 down-regulated genes.Enrichment analysis showed that the common differentially expressed genes mainly concentrated in the immune response,protein binding,and the plasma membrane,mainly involved in cytokine-cytokine receptor interaction,hematopoietic cell lineage,T cell receptor signa⁃ling pathway,natural killer cell-mediated cytotoxicity and transcriptional dysregulation in cancer.The protein-interaction net⁃work suggested that there were 3 main gene clusters and 10 key genes,including CD2,CCR7,PRF1,CD247,GZMB,GZMA,KLRB1,CD3D,IL2RB and CCL5.Conclusion The key genes and signaling pathways selected will help to deepen the under⁃standing of the pathogenesis of adult AML and provide a theoretical basis for subsequent studies.