低氧环境下microRNA-1179的表达对三阴性乳腺癌增殖和迁移的影响

Hypoxia promote the triple negative breast cancer proliferation and migration via regulating miR⁃1179

目的 观察微小RNA1179(microRNA-1179,miR-1179)在三阴性乳腺癌中的表达以及对三阴性乳腺癌增殖和迁移的影响。方法 qPCR检测miR-1179在三阴性乳腺癌和癌旁乳腺组织以及三阴性乳腺癌细胞株和正常乳腺细胞株中的表达情况。通过转染miR-1179类似物过表达miR-1179,在低氧微环境和正常氧环境中,对三阴性细胞株进行培养,通过CCK-8实验、划痕实验研究细胞生物学行为。结果 在三阴性乳腺癌组织和细胞株MDA-MB-231中miR-1179的表达明显低于癌周乳腺组织和正常的细胞株MCF-10A。在低氧环境中,MDA-MB-231在肿瘤组织中的表达明显降低,细胞的增殖和迁移能力增强,而miR-1179过表达后逆转了低氧引起的细胞增殖和迁移能力。结论 低氧可以通过负性调控miR-1179而促进细胞的增殖和迁移。

Objective To illustrate the expression,function and mechanism of microRNA⁃1179(miR⁃1179)in triple negative breast cancer.Methods The qPCR was used to show the expression of miR⁃1179 in the tumor tissues,adjacent normal tissues and MCF⁃10A cells.The overexpression of miR⁃1179 was completed via the transfection of miR⁃1179 mimic in MDA⁃MB⁃231 cells.The CCK⁃8 and would healing assay were used to analyze the cell proliferation and migration.Results Hypoxia could significantly decrease the expression of miR⁃1179 and enhance the cell proliferation and migration.However,the enhanced function induced by hypoxia was reversed by miR⁃1179 overex⁃pression.Conclusion Hypoxia could enhance the triple negative cancer proliferation and migration via miR⁃1179.