半糯粳稻与常规粳稻籽粒代谢物的差异及氨基酸合成

Grain Metabolite Differences and Amino Acid Synthesis of Semi-glutinous Japonica Rice and Conventional Japonica Rice

为探明半糯粳稻与常规粳稻代谢组分的差异以及代谢物在氨基酸合成通路中的变化。本研究以1个半糯粳稻品种‘扬农香28’(YNX28H)和2个常规粳稻品种‘扬大3号’(YD3)、‘扬大4号’(YD4)为试验材料,利用LC-MS非靶向代谢组学技术检测了3个水稻品种的代谢物。结果表明,YNX28H和YD3比较组中鉴定了201种差异代谢物(120个上调和81个下调),YNX28H和YD4比较组中鉴定了246种差异代谢物(120个上调和126个下调)。半糯粳稻品种‘扬农香28’含特有代谢物89种。半糯粳稻与常规粳稻的差异代谢物主要涉及氨基酸代谢通路、脂类代谢通路和淀粉合成通路。YNX28H和YD3比较组氨基酸合成通路中L-天冬氨酸、L-天门冬酰胺、L-谷氨酸、精氨酸、氨气代谢物显著上升。YNX28H和YD4比较组氨基酸合成通路中丝氨酸、L-天冬氨酸、L-天门冬酰胺、L-谷氨酸、氨气代谢物显著上升。这些代谢物在半糯粳稻氨基酸合成与代谢过程中扮演者重要的角色。本研究为培育优质食味粳稻新品种提供理论依据。

To explore the difference of metabolic components between semi-glutinous japonica rice and conventional japonica rice and the changes of metabolites in the amino acid synthesis pathway.In this study,one semi-glutinous japonica rice variety'Yangnongxiang28'(YNX28H)and two conventional japonica rice varieties'Yangda3'(YD3)and'Yangda4'(YD4)were used as experimental materials,and LC-MS non-targeted metabonomics technique was used to detect metabolites of three rice varieties.The results showed that 201 differential metabolites(DMs)(120 up-regulated and 81 down-regulated)were identified in YNX28H and YD3 comparison groups,and 246 DMs(120 up-regulated and 126 down-regulated)were identified in YNX28H and YD4 comparison groups.'Yangnongxiang28',a semi-glutinous japonica rice variety,contains 89 specific metabolites.The DMs of semi-glutinous japonica rice and conventional japonica rice are mainly involved in amino acid metabolism pathway,lipid metabolism pathway and starch synthesis pathway.In YNX28H and YD3 comparison groups,L-aspartate,L-asparagine,L-glutamate,arginine and NH3 metabolites in the amino acid synthesis pathway increased significantly.In YNX28H and YD4 comparison groups,serine,L-aspartate,L-asparagine,L-glutamate and NH3 metabolites in the amino acid synthesis pathway increased significantly.These metabolites play an important role in the amino acid synthesis and metabolism of semi-glutinous japonica rice.This study provides a theoretical basis for the cultivation of new varieties of high-quality and tasty japonica rice.