抗F2R抗体对小鼠口腔鳞癌细胞移植瘤的治疗作用和免疫学机制

The treatment effect of antibody F2R on the mice bearing oral squamous cell carcinoma and the underlying immune mechanism

目的 观察凝血因子2凝血酶受体(coagulation factor 2 thrombin receptor,F2R)抗体对小鼠口腔鳞癌细胞移植瘤生长抑制及对肿瘤、脾脏组织中髓源性抑制细胞(myeloid-derived suppressor cells,MDSCs)的影响。方法 通过皮下注射小鼠SCC-7细胞构建小鼠皮下移植瘤模型,实验组给予抗F2R抗体治疗,对照组注射与治疗组相同体积生理盐水。通过流式细胞仪分析移植瘤和脾脏组织中CD11b+Gr-1+MDSCs细胞比例。结果 抗F2R抗体治疗后,小鼠皮下移植瘤体积和重量明显降低,抑瘤率达66.7%(P<0.0001)。在小鼠皮下移植瘤抗F2R抗体组中,MDSCs细胞比例较对照组显著降低,分别为(0.876±0.259)%和(3.814±1.525)%(P<0.01)。而在小鼠脾脏组织抗F2R抗体组中,MDSCs细胞比例较对照组无显著差异。结论 抗F2R抗体治疗能抑制小鼠口腔鳞癌移植瘤模型肿瘤的进展,降低移植瘤组织中CD11b+Gr-1+MDSCs的比例。

Objective To investigate the influence of coagulation factor 2 thrombin receptor(F2R)on the inhibition of tumor and the percentage of myeloid-derived suppressor cells(MDSCs)of tumor and spleen tissues in mice bearing oral squamous cell carcinoma.Methods The C3H xenograft model was set up through subcutaneously injecting mouse with oral squamous cell carcinomas cells SCC-7.The experimental group was treated with antibody F2R,and the control group was injected with normal saline.Flow cytometry was used to analyze the proportion of CD11b+Gr-1+MDSCs cells in the tumor and spleens of the C3H xenograft model.Results After therapy of antibody F2R,the tumor volume and weight in xenograft mice model were reduced significantly,and the tumor inhibition rate reached to 66.7%(P<0.0001).The flow cytometry showed that in the xenograft mice model,the proportion of MDSCs cells of antibody F2R group was significantly lower than that of the control group in tumor tissues(P<0.01).However,in the spleen tissues,there was no significant difference between the antibody F2R treatment group and the control group.Conclusions Antibody F2R treatment could inhibit the tumor progression in the xenograft model and reduce the proportion of CD11b+Gr-1+MDSCs cells in tumor tissues.