广东省东莞市茶山镇健康人外周血T淋巴细胞分布的研究

Study of distribution of peripheral T-lymphocytes in healthy adults from Chashan Town of Dongguan in Guangdong Province

目的分析广东省东莞市茶山镇健康成人外周静脉血T淋巴细胞亚群的分布。方法收集827名健康成人静脉全血标本,使用流式细胞术检测CD3^(+)T淋巴细胞、CD4^(+)T淋巴细胞、CD8^(+)T淋巴细胞、CD4^(+)CD8^(+)双阳性T淋巴细胞、调节性T淋巴细胞(Treg)的比例和CD4^(+)/CD8^(+)T淋巴细胞比值。结果CD3^(+)T淋巴细胞、CD4^(+)T淋巴细胞、CD8^(+)T淋巴细胞、CD4^(+)/CD8^(+)T淋巴细胞比值、双阳性T淋巴细胞和Treg细胞的比例分别为40.5%~75.2%、35.4%~68.9%、27.4%~61.8%、0.6~2.4、0.2%~2.7%和2.4%~6.0%。各淋巴细胞亚群的分布在不同年龄、性别间分布比较差异有统计学意义(P均<0.05)。结论本研究成功分析了广东省东莞市茶山镇健康成人T淋巴细胞亚群的分布,可为本地区临床医师提供有价值的参考依据。

Objective To explore the distribution of T lymphocyte subsets in peripheral blood of healthy adults from Chashan Town of Dongguan in Guangdong Province.Methods The venous whole blood samples were collected from 827 healthy adults.The percentage of CD3+T cells,CD4+T cells,CD8+T cells,CD4+CD8+double positive(DP)T cells,and regulatory T(Treg)cells and CD4+/CD8+ratio were determined by flow cytometry.Results The percentage of CD3+T cells,CD4+T cells,CD8+T cells,CD4+/CD8+ratio,DP T cells and Treg cells were determined as 40.5%-75.2%,35.4%-68.9%,27.4%-61.8%,0.6-2.4,0.2%-2.7%and 2.4%-6.0%,respectively.Significant differences were observed in the distribution of lymphocyte subsets regarding age and gender(all P<0.05).Conclusion The distribution of T lymphocyte subsets in healthy adults from Chashan Town of Dongguan in Guangdong Province was successfully analyzed,providing valuable reference for clinicians in this region.