含氯生物活性玻璃支架的制备及性能研究

Study on preparation and properties of chlorine containing bioactive glass scaffold

目的:研究CaCl_(2)取代CaO对多孔生物活性玻璃(Bioactive glasses,BG)支架的力学性能及生物活性的影响。方法:以含磷玻璃(SiO_(2)-ZnO-CaO-Na_(2)O-P_(2)O_(5)-Si_(3)N_(4))为基础,用不同含量(4 wt%、8 wt%、12 wt%)的CaCl_(2)取代上述CaO,选用熔融法制取基础玻璃,而后以聚氨酯(Polyurethane,PU)海绵为模板,选用有机泡沫浸渍工艺制取含氯的BG支架,测定抗弯强度(Bending strength,BS)、抗压强度(Compressive strength,CS)、降解性能、矿化活性。结果:用CaCl_(2)取代CaO后,C(不含CaCl_(2))、C1(含4 wt%CaCl_(2))、C2(含8 wt%CaCl_(2))、C3(含12 wt%CaCl_(2))四组的CS与BS均逐渐下降,经统计学分析差别有统计学意义(P<0.05),两两比较,结果为六个对比组间均有差异(均P<0.05);C、C1、C2、C3四组在模拟体液浸泡24 h后的失重比递增,经统计学分析差别有统计学意义(P<0.05),两两比较,结果为六个对比组间均有差异(均P<0.05);扫描电镜图片和XRD分析的结果显示,在模拟体液浸泡24 h后,C、C1、C2、C3四组均有矿化活性,且逐渐递增。结论:CaCl_(2)会降低多孔BG支架的力学性能,其质量百分比应控制在12 wt%以下为宜,此外,其还可提高多孔BG支架的生物活性。

Objective:To study the effects of CaCl_(2)replacing CaO on the mechanical performance and biological activity of porous bioactive glass(BG)scaffolds.Methods:Based on phosphorus-containing glass(SiO_(2)-ZnO-CaO-Na_(2)O-P_(2)O_(5)-Si_(3)N_(4)),the above CaO was replaced by different contents of CaCl_(2)(4 wt%,8 wt%,12 wt%),and the base glass was prepared by melting method,and then the PU sponge was used as the template,and the organic foam impregnation process was used to prepare the chlorine-containing BG scaffold,and the bending strength(BS),compressive strength(CS),degradation performance and mineralization activity were measured.Results:When CaO was replaced with CaCl_(2),the CS and BS of C(without CaCl_(2)),C1(containing 4 wt%CaCl_(2)),C2(containing 8 wt%CaCl_(2))and C3(containing 12 wt%CaCl_(2))groups decreased gradually and the difference in statistical analysis was significant(P<0.05),after pairwise comparison,the results showed that there were differences among the six comparison groups(all P<0.05);The weight loss ratio of group C,C1,C2 and C3 gradually increased after immersion in simulated body fluid for 24 hours,and the difference in statistical analysis was statistically significant(P<0.05),after pairwise comparison,the results showed that there were differences among the six comparison groups(all P<0.05);After immersion in simulated body fluid for 24 hours,the four groups C,C1,C2,and C3 all had mineralization activity,and it gradually increased.Conclusion:CaCl_(2)can reduce the mechanical properties of the porous BG scaffolds,and its mass percentage should be controlled below 12 wt%;In addition,it can also improve the biological activity of the porous BG scaffolds.